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We present several novel applications of single molecule localization to both proteomics and genomics, including a method for improving the resolving capacity by more than an order of magnitude (Single Molecule High Resolution Imaging with Photo-bleaching, or SHRImP), a method for extending wide field single-molecule localization to three dimensions (Three Dimensional Imaging with One Nanometer Accuracy, or 3IONA), and applications of these techniques to problems in genomics and molecular motors.","abstract_html":"Fluorescence single molecule localization has become a powerful tool for investigating problems in biological physics. However, there are numerous limitations on the types of measurements that can be made with typical instrument designs based on objective-type TIR and wide field CCD imaging. We present several novel applications of single molecule localization to both proteomics and genomics, including a method for improving the resolving capacity by more than an order of magnitude (Single Molecule High Resolution Imaging with Photo-bleaching, or SHRImP), a method for extending wide field single-molecule localization to three dimensions (Three Dimensional Imaging with One Nanometer Accuracy, or 3IONA), and applications of these techniques to problems in genomics and molecular motors.","abstract_has_math":false,"creators":["Gordon, Matthew Paul"],"institution":null,"degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Physics","degree_department":null,"school":null,"contributors":["Selvin, Paul R."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-10-12T22:43:09Z","date_published":"2012-10-12T22:43:09Z","updated_at":"2026-07-22T22:25:31Z","subjects":["Fluorescence Spectroscopy","Genetic Haplotypes","Photobleaching"],"languages":["en"],"rights":["©2006 Matthew Paul Gordon"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["5639998"],"render_values":[{"text":"5639998","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/34737","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Selvin, Paul R."]},{"key":"dc:creator","label":"Author","values":["Gordon, Matthew Paul"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2012-10-12T22:43:09Z","10000-01-01","2006-10"]},{"key":"dc:type","label":"Dc Type","values":["Dissertation / Thesis","text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physics"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Fluorescence Spectroscopy","Genetic Haplotypes","Photobleaching"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["©2006 Matthew Paul Gordon"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["5639998","http://hdl.handle.net/2142/34737"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Fluorescence single molecule localization has become a powerful tool for investigating problems in biological physics. 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However, there are numerous limitations on the types of measurements that can be made with typical instrument designs based on objective-type TIR and wide field CCD imaging. We present several novel applications of single molecule localization to both proteomics and genomics, including a method for improving the resolving capacity by more than an order of magnitude (Single Molecule High Resolution Imaging with Photo-bleaching, or SHRImP), a method for extending wide field single-molecule localization to three dimensions (Three Dimensional Imaging with One Nanometer Accuracy, or 3IONA), and applications of these techniques to problems in genomics and molecular motors.","Submitted by Meng Tao (mengtao2@illinois.edu) on 2012-10-12T22:43:09Z No. of bitstreams: 1 Gordon_Matthew.pdf: 1409796 bytes, checksum: 39e18fee33746bd9ad27d78aaecd0446 (MD5)","Made available in DSpace on 2012-10-12T22:43:09Z (GMT). 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