A microfluidic platform for hematopoietic stem cell separation

Microfluidics manipulates, processes, and controls small volumes of fluids in channels or compartments with cross-sectional dimensions on the order of 10-100 µm. By integrating functional components such as channels, actuators, and sensors, and by leveraging characteristic physical phenomena occurring at the microscale, microfluidics brings microfabrication, chemistry and physics together to develop new experimental paradigm for broad applications, including the study of biological problems. Indeed, microfluidics operates at a scale that is particularly suitable for biological sample analysis, which provides great opportunities for technology innovation in bioanalytical assays and biomedical applications such as point-of-care devices. Challenges due to the complexity of the biological system can be overcome by introducing new engineering elements and performing systematic device optimization. In this work, a microfluidic immunoaffinity based silicon micropost device for hematopoietic stem cell enrichment is discussed, which demonstrates how innovations in microfluidics can revolutionize current clinical therapies. Appropriate surface chemistry for robust immobilization of proteins on the surface of different materials and topography was developed. Silicon chips with three-dimensional features, arrays of microposts in a wide range of arrangements, were designed. The fluid dynamics in the various micropost arrays was modeled and optimized to meet specific needs of cell enrichment for clinical application. The optimized three-dimensional micropost devices achieve hematopoietic stem cell enrichment at a level that outperforms all current technologies and successfully meets the requirements for clinical transplantation applications.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2012

Author dc:creator

• Zhang, Jieqian

Contributors dc:contributor

• Kenis, Paul J.A.
• Leckband, Deborah E.
• Gaskins, H. Rex
• Bailey, Ryan C.

• Microfluidics
• cell enrichment
• hematopoietic stem cell

Statement dc:rights

• Copyright 2012 Jieqian Zhang

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/32058

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/32058