Characteristics and applications of the enhanced HRP-catalyzed oxidation of luminol

Chemiluminescence (CL) is the process in which the energy from a chemical reaction is released and thus produces an electronically excited species which, upon decay to the ground state, emits light. One of the most efficient chemiluminescent molecules is luminol. As a result of this, luminol has received much attention in chemical analysis. An analytically useful CL system which has also received much attention in recent years is the enhanced horseradish peroxidase (HRP)-catalyzed luminol reaction. Generally, the enhancers are halogenated phenols or aromatic amines. Unfortunately, only limited work has been done to characterize the action of these enhancers. The work reported to date has been empirical characterization of enhanced CL oxidations with regards to enhancer identity and concentration, pH, CL molecule, oxidants, HRP isoenzyme composition and the CL emission spectrum. This research project studied the p-iodophenol enhanced HRP-catalyzed oxidation of luminol improve the understanding of this reaction. The rate and total yield of photon emission, the rate and extent of luminol oxidation, $\phi\sb{\rm CL}$ (quantum efficiency of chemiluminescence), $\phi\sb{\rm fl}$ (quantum efficiency of fluorescence) and $\phi\sb{\rm ex}$ (quantum efficiency of excitation) were determined for this reaction under a variety of conditions. In addition, compounds with structural similarity to known enhancers were studied via flow injection analysis (FIA) to determine if a correlation between structure and enhancer ability could be made.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry, General

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Gilbert, Brian Douglas

• Chemistry, General
• Chemistry, Analytical
• Chemistry, Biochemistry

Statement dc:rights

• Copyright 1989 Gilbert, Brian Douglas

Language dc:language

eng

Identifier

AAI9010862
(UMI)AAI9010862

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/23642