{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/23642"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/23642","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characteristics and applications of the enhanced HRP-catalyzed oxidation of luminol","abstract":"Chemiluminescence (CL) is the process in which the energy from a chemical reaction is released and thus produces an electronically excited species which, upon decay to the ground state, emits light. One of the most efficient chemiluminescent molecules is luminol. As a result of this, luminol has received much attention in chemical analysis. An analytically useful CL system which has also received much attention in recent years is the enhanced horseradish peroxidase (HRP)-catalyzed luminol reaction. Generally, the enhancers are halogenated phenols or aromatic amines. Unfortunately, only limited work has been done to characterize the action of these enhancers. The work reported to date has been empirical characterization of enhanced CL oxidations with regards to enhancer identity and concentration, pH, CL molecule, oxidants, HRP isoenzyme composition and the CL emission spectrum. This research project studied the p-iodophenol enhanced HRP-catalyzed oxidation of luminol improve the understanding of this reaction. The rate and total yield of photon emission, the rate and extent of luminol oxidation, $\\phi\\sb{\\rm CL}$ (quantum efficiency of chemiluminescence), $\\phi\\sb{\\rm fl}$ (quantum efficiency of fluorescence) and $\\phi\\sb{\\rm ex}$ (quantum efficiency of excitation) were determined for this reaction under a variety of conditions. In addition, compounds with structural similarity to known enhancers were studied via flow injection analysis (FIA) to determine if a correlation between structure and enhancer ability could be made.","abstract_html":"Chemiluminescence (CL) is the process in which the energy from a chemical reaction is released and thus produces an electronically excited species which, upon decay to the ground state, emits light. One of the most efficient chemiluminescent molecules is luminol. As a result of this, luminol has received much attention in chemical analysis. An analytically useful CL system which has also received much attention in recent years is the enhanced horseradish peroxidase (HRP)-catalyzed luminol reaction. Generally, the enhancers are halogenated phenols or aromatic amines. Unfortunately, only limited work has been done to characterize the action of these enhancers. The work reported to date has been empirical characterization of enhanced CL oxidations with regards to enhancer identity and concentration, pH, CL molecule, oxidants, HRP isoenzyme composition and the CL emission spectrum. This research project studied the p-iodophenol enhanced HRP-catalyzed oxidation of luminol improve the understanding of this reaction. The rate and total yield of photon emission, the rate and extent of luminol oxidation, $\\phi\\sb{\\rm CL}$ (quantum efficiency of chemiluminescence), $\\phi\\sb{\\rm fl}$ (quantum efficiency of fluorescence) and $\\phi\\sb{\\rm ex}$ (quantum efficiency of excitation) were determined for this reaction under a variety of conditions. In addition, compounds with structural similarity to known enhancers were studied via flow injection analysis (FIA) to determine if a correlation between structure and enhancer ability could be made.","abstract_has_math":true,"creators":["Gilbert, Brian Douglas"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry, General","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T14:21:41Z","date_published":"2011-05-07T14:21:41Z","updated_at":"2026-07-22T22:25:22Z","subjects":["Chemistry, General","Chemistry, Analytical","Chemistry, Biochemistry"],"languages":["eng"],"rights":["Copyright 1989 Gilbert, Brian Douglas"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9010862","(UMI)AAI9010862"],"render_values":[{"text":"AAI9010862","href":null,"code":true},{"text":"(UMI)AAI9010862","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/23642","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Gilbert, Brian Douglas"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T14:21:41Z","10000-01-01","1989"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry, General","Chemistry, Analytical","Chemistry, Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, General","Chemistry, Analytical","Chemistry, Biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1989 Gilbert, Brian Douglas"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9010862","(UMI)AAI9010862","http://hdl.handle.net/2142/23642"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Chemiluminescence (CL) is the process in which the energy from a chemical reaction is released and thus produces an electronically excited species which, upon decay to the ground state, emits light. One of the most efficient chemiluminescent molecules is luminol. As a result of this, luminol has received much attention in chemical analysis. An analytically useful CL system which has also received much attention in recent years is the enhanced horseradish peroxidase (HRP)-catalyzed luminol reaction. Generally, the enhancers are halogenated phenols or aromatic amines. Unfortunately, only limited work has been done to characterize the action of these enhancers. The work reported to date has been empirical characterization of enhanced CL oxidations with regards to enhancer identity and concentration, pH, CL molecule, oxidants, HRP isoenzyme composition and the CL emission spectrum. This research project studied the p-iodophenol enhanced HRP-catalyzed oxidation of luminol improve the understanding of this reaction. The rate and total yield of photon emission, the rate and extent of luminol oxidation, $\\phi\\sb{\\rm CL}$ (quantum efficiency of chemiluminescence), $\\phi\\sb{\\rm fl}$ (quantum efficiency of fluorescence) and $\\phi\\sb{\\rm ex}$ (quantum efficiency of excitation) were determined for this reaction under a variety of conditions. In addition, compounds with structural similarity to known enhancers were studied via flow injection analysis (FIA) to determine if a correlation between structure and enhancer ability could be made.","It was found that the photon emission, the rate and extent of reaction increased in the presence of p-iodophenol. However, this enhancer had little effect on $\\phi\\sb{\\rm CL}$, $\\phi\\sb{\\rm fl}$ and $\\phi\\sb{\\rm ex}$. Thus, it seems that the major means of enhancement is an acceleration of the luminol reaction. The FIA studies of various phenols and amines showed that most candidate compounds produced suppression of the HRP-catalyzed oxidation of luminol. However, this procedure led to the discovery of a new enhancing compound (4-chloro-3-methyl phenol). This screening also allowed some structural correlations to be made (such as position and type of substitution on the phenolic ring). Optimization of this FIA system with respect to HRP concentration, luminol concentration, H$\\sb2$O$\\sb2$ concentration and the observation time (i.e., flow rate) allowed detection of p-iodophenol as low as 670 nM.","Made available in DSpace on 2011-05-07T14:21:41Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9010862.pdf: 6458233 bytes, checksum: 9eaa4b8d58d15177caae5ab19c2b0930 (MD5) Previous issue date: 1989","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:05:51Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:31:35-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Characteristics and applications of the enhanced HRP-catalyzed oxidation of luminol"]}]}],"canonical_facts":{"dc:creator":["Gilbert, Brian Douglas"],"dc:date":["2011-05-07T14:21:41Z","10000-01-01","1989"],"dc:description":["Chemiluminescence (CL) is the process in which the energy from a chemical reaction is released and thus produces an electronically excited species which, upon decay to the ground state, emits light. One of the most efficient chemiluminescent molecules is luminol. As a result of this, luminol has received much attention in chemical analysis. An analytically useful CL system which has also received much attention in recent years is the enhanced horseradish peroxidase (HRP)-catalyzed luminol reaction. Generally, the enhancers are halogenated phenols or aromatic amines. Unfortunately, only limited work has been done to characterize the action of these enhancers. The work reported to date has been empirical characterization of enhanced CL oxidations with regards to enhancer identity and concentration, pH, CL molecule, oxidants, HRP isoenzyme composition and the CL emission spectrum. This research project studied the p-iodophenol enhanced HRP-catalyzed oxidation of luminol improve the understanding of this reaction. The rate and total yield of photon emission, the rate and extent of luminol oxidation, $\\phi\\sb{\\rm CL}$ (quantum efficiency of chemiluminescence), $\\phi\\sb{\\rm fl}$ (quantum efficiency of fluorescence) and $\\phi\\sb{\\rm ex}$ (quantum efficiency of excitation) were determined for this reaction under a variety of conditions. In addition, compounds with structural similarity to known enhancers were studied via flow injection analysis (FIA) to determine if a correlation between structure and enhancer ability could be made.","It was found that the photon emission, the rate and extent of reaction increased in the presence of p-iodophenol. However, this enhancer had little effect on $\\phi\\sb{\\rm CL}$, $\\phi\\sb{\\rm fl}$ and $\\phi\\sb{\\rm ex}$. Thus, it seems that the major means of enhancement is an acceleration of the luminol reaction. The FIA studies of various phenols and amines showed that most candidate compounds produced suppression of the HRP-catalyzed oxidation of luminol. However, this procedure led to the discovery of a new enhancing compound (4-chloro-3-methyl phenol). This screening also allowed some structural correlations to be made (such as position and type of substitution on the phenolic ring). Optimization of this FIA system with respect to HRP concentration, luminol concentration, H$\\sb2$O$\\sb2$ concentration and the observation time (i.e., flow rate) allowed detection of p-iodophenol as low as 670 nM.","Made available in DSpace on 2011-05-07T14:21:41Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9010862.pdf: 6458233 bytes, checksum: 9eaa4b8d58d15177caae5ab19c2b0930 (MD5) Previous issue date: 1989","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:05:51Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:31:35-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9010862","(UMI)AAI9010862","http://hdl.handle.net/2142/23642"],"dc:language":["eng"],"dc:rights":["Copyright 1989 Gilbert, Brian Douglas"],"dc:subject":["Chemistry, General","Chemistry, Analytical","Chemistry, Biochemistry"],"dc:title":["Characteristics and applications of the enhanced HRP-catalyzed oxidation of luminol"],"dc:type":["text"],"thesis:degree_discipline":["Chemistry, General","Chemistry, Analytical","Chemistry, Biochemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:22Z"}