Interrelationships among estrogen, insulin-like growth factor-I and cyclic adenosine monophosphate in the regulation of uterine progesterone and estrogen receptors

Studies were undertaken to examine regulation of progesterone receptor gene expression and estrogen receptor bioactivity in uterine cells, and to determine the cellular mechanisms through which various factors mediate regulation of these receptor proteins. Exposure of uterine cells maintained in primary culture to estradiol, IGF-I, or to agents which raised intracellular cAMP levels stimulated significant increases in progesterone receptor (PR) content. Stimulation of PR levels by these agents was suppressed equally by co-treatment with antiestrogen (ICI 164,384) or protein kinase inhibitors, or by pre-treatment of cells with actinomycin D or cycloheximide. These results suggest that regulation of PR occurs at the level of the PR gene, and that induction of PR stimulated by these agents requires activation of estrogen receptor (ER) and the action of protein kinases. Treatment with cGMP and activators of protein kinase C failed to alter the basal level of PR, or the induction of PR stimulated by other agents.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biology, Molecular

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Aronica, Susan Marie

Contributors dc:contributor

• Katzenellenbogen, Benita S.

• Biology, Molecular
• Biology, Cell
• Biology, Animal Physiology

Statement dc:rights

• Copyright 1994 Aronica, Susan Marie

Language dc:language

eng

Identifier

AAI9416335
(UMI)AAI9416335

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/23557