A genetic analysis of bacteriophage lambda integrase-core site interactions

Bacteriophage lambda utilizes a site-specific recombination system to integrate its DNA into the E. coli chromosome during lysogeny. The viral protein, Integrase (Int), catalyzes strand exchange between the phage attachment site, attP, and the host target sequence, attB to generate the prophage sites, attL and attR. attP contains the core region, where strand exchange occurs, and the required flanking sequences, the P and P$\sp\prime$ arms. Within the core, there are two Int core-type binding sites. The flanking arm sequences contain binding sites for Int as well as for IHF, Xis, and FIS. This array of protein-DNA interactions forms a higher-order complex, the intasome, which is the active substrate for recombination.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• MacWilliams, Maria Patrice

Contributors dc:contributor

• Gardner, Jeffrey F.

• Biology, Molecular
• Biology, Genetics
• Biology, Microbiology

Statement dc:rights

• Copyright 1992 MacWilliams, Maria Patrice

Language dc:language

eng

Identifier

AAI9236529
(UMI)AAI9236529

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/23413