Analysis of organ-specific gene expression in soybean

Organ specificity is an important criterion to be considered for the expression of foreign genes in plants. For crop improvement by genetic engineering it is critical to introduce genes in specific organs that can be expressed at an appropriate developmental stage. This study involved the analysis of organ-specific control conferred by 5$\sp\prime$ and 3$\sp\prime$ regulatory sequences of the seed-lectin gene of soybean (Glycine max) on the expression of a foreign gene. The reporter gene β-glucuronidase (GUS), was linked to the 5$\sp\prime$ and 3$\sp\prime$ sequences of the lectin gene and transferred into Arabidopsis thaliana and Nicotiana tabacum. GUS activity assayed on transformed tissues revealed seed-specific expression of GUS under control of the lectin promoter. This expression pattern was found to be conserved among transformed Arabidopsis, and tobacco. These results confirmed that the soybean seed lectin has a strong seed-specific promoter which could be used to successfully drive seed-specific expression of foreign genes.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Sundararaman, Vijaya Priya

Contributors dc:contributor

• Vodkin, Lila O.

• Biology, Molecular
• Biology, Genetics

Statement dc:rights

• Copyright 1996 Sundararaman, Vijaya Priya

Language dc:language

eng

Identifier

9780591089240
AAI9702676
(UMI)AAI9702676

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/23410