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Showing 1 to 20 of 143 for “"GUS"”.
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Totality of experience: sound, music and mythology in Gus Van Sant’s “Death Quartet”
Reflecting on Gus Van Sant’s films Gerry (2003) Elephant (2004) and Last Days (2005), the director’s long-term sound-designer Leslie Shatz observed that “You have to get into the totality of the experience and not just the dialogue”. Shatz’s comment expresses something fundamental about the …
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Expression of Gus Gene in Tobacco (Nicotiana tabacum) and Corn (Zea mays) Using Dimboa-Resistant Agrobacterium Strains
… tumefaciens, bearing binary plasmids pGUS and pBG5. These plasmids contained reporter GUS (p-glucuronidase) gene and NPT II (Neomycin Phosphotransferase) gene conferring resistance to kanamycin. These genes were placed under the eukaryotic 35S CaMV promoter and had a NOS terminator. A …
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The Role of Devotion (mos gus) in Tibetan Heart Essence Traditions: Devotional Affect and Its Relationship to Dzogchen’s Foundational Practices (rdzogs chen)
The Role of Devotion (mos gus) in Tibetan Heart Essence Traditions: Devotional Affect and Its Relationship to Dzogchen’s Foundational Practices (rdzogs chen) examines how devotion, faith (dad pa), and awakened mind (byang chub kyi sems) function philosophically and epistemologically and in …
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Rechtspolitische und rechtliche Probleme der Zuwanderung
… und der Integration von Zuwanderern aus den GUS-Staaten in Deutschland ist eine bedeutsame politische und rechtliche Thematik. Diese Thematik ist in Deutschland bisher noch wenig untersucht und nur in Teilbereichen bearbeitet. Deshalb untersucht das vorgestellte Werk folgende Fragen und …
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Factors influencing transient gene expression in electroporated tall fescue (Festuca arundinacea Schreb.) protoplasts
… protoplasts, after the reporter β-glucuronidase (GUS) gene was introduced by electroporation. In a time-course study of transient gene expression, GUS activity peaked at 24 h after electroporation. Among the different field strength conditions tested, maximum GUS activity was observed at 750 V/cm. …
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Applications of RAPD Markers in Soybean: Genetic Diversity and Linkage Analysis
… procedure plasmids carrying the bar and gus genes were applied to cut stigmas. Approximately 5000 seeds were produced from treated flowers. No plants derived from seeds produced by flowers treated with the plasmid carrying the bar gene were found to be as herbicide resistant as the …
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Analysis of ABA-regulated expression of the maize Glb1 gene in tobacco seeds and maize cells
… P3377 cells. For transgenic studies, a ppGlb1GUS construct, consisting of 1402 bp 5$\sp\prime$ flanking sequence of Glb1, 1919 bp GUS (coding sequence, and 283 bp 3$\sp\prime$ Nos terminator, was cloned into Bin19 vector and introduced into tobacco plants by Agrobacterium-mediated …
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Agrobacterium-mediated gene transfer into kiwifruit
… that express a chimaeric β-D-glucuronidase (GUS) gene in plants cells but not in bacterial cells. This feature allows GUS activity from transformed plant cells to be assayed in the presence of Agrobacterium. Preliminary experiments examined the expression of these chimaeric GUS genes in …
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In Vitro Regeneration and Genetic Transformation of Sesbania Drummondii: A Medicinally and Environmentally Important Plant
… for S. drummondii. This plasmid contains a GUS (P-glucuronidase) reporter gene and a gene conferring resistance to the antibiotic hygromycin. The genes are placed under the control of the 35S CaMV (cauliflower mosaic virus) promoter and a NOS terminator. A plant catalase intron is inserted …
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Analysis of organ-specific gene expression in soybean
… gene. The reporter gene $\beta$-glucuronidase (GUS), was linked to the 5$\sp\prime$ and 3$\sp\prime$ sequences of the lectin gene and transferred into Arabidopsis thaliana and Nicotiana tabacum. GUS activity assayed on transformed tissues revealed seed-specific expression of GUS under control of …
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An approach for upscaling the flow effects of multiple deep-water genetic units
… of geological bodies, termed as genetic units (GUs). The data collection is difficult and costly in both economic and time aspects. Understanding the role of the interactions between GUs could play a major role in helping to simplify the highly heterogeneous complex structures. The aim of this …
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Farnesyltransferase: Gene Expression in Plants and Role in Plant Development
… sequence (~2 kb) of PsFTa was fused to GUS (b-glucuronidase) and GFP (green fluorescent protein) reporter genes and introduced into tobacco plants. This 2 kb upstream region appears insufficient to provide PsFTa promoter function. On the other hand, 3.2 kb of PsFTb 5'-upstream sequence …
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Promoter analysis in transgenic sugar beet
… reporter gene which encodes P-D-glucuronidase (GUS) to examine promoters in transgenic sugar beet. GUS activities were measured in eight replicates of each of eight sugar beet hairy root clones which had been obtained after inoculation of material grown from a single seed with a CaMV 35S …
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Stèidheachadh Ghnàthan-cànain Ùra Cuairteachadh Ideòlais-chànain ann an Trì Gaeltachtaí Nua
… de dhealbhadh-cànain ann am buidhnean beaga agus aig a' mhion-ìre: Coimhearsnachd Bhóthar Seoighe ann am Béal Feirste, Èirinn a Tuath, Scéim Chónaithe Choláiste na Tríonóide ann am Baile Átha Cliath, Poblach na h-Èireann agus Sgìre Charn Tóchair ann an Contae Dhoire a Deas, Èirinn a Tuath. …
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Expression of atrial natriuretic factor in transgenic potatoes
… expression of cytosolic beta-glucuronidase (GUS) to confirm the functionality of the DNA regulatory sequences used. All six chimaeric gene fusions were cloned into the binary vector, pGA643, or its derivative. These were then introduced into potato plants via leaf disc transformation using …
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Tissue and Cell-Type Localization and Partial Characterization of a Xylem Papain-Type Cysteine Protease From Arabidopsis
… restricted to xylem. Cell-type localization of GUS expression under the control of a putative XCP1 promoter indicated that the XCP1 promoter specifies expression of XCP1 in tracheary elements in leaves, stems, roots and flowers. XCP1 promoter-driven GUS activity was not associated with senescing …
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Regulação do promotor de GmNAC081 durante o desenvolvimento de Nicotiana tabacum e em resposta a estresses abióticos
… pb, 750 pb e 500 bp fusionadas ao gene repórter GUS. Análises fluorimétricas quantitativas de GUS revelaram a presença de elementos em cis responsivos a PEG na regiao contida pelas posições -1000 a -750. Pelo menos dois elementos responsivos a tunicamincina devem estar contidos nas regioes -1000 …
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Characterization of a cold-responsive dehydrin promoter
… for cold induction of AtERD14, AtERD14prom::GFP/GUS and AtERD14prom::AtERD14 in AtERD14 KO constructs were created and stably transformed into A. thaliana. Analysis of the constructs showed the AtERD14 promoter alone was insufficient to respond to cold, and it was necessary to attach the AtERD14 …
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ORMOCER (inorganic-organic hybrid polymer)-zeolite Nanocomposites: Advanced Membrane Materials for Gas Separation
… cerine-1,3-dimethacrylateurethanetriethoxysilane (GUS)-based ORMOCER® and dimethylsiloxane modified GUS-ORMOCER® resins were used for the fabrication of freestanding mixed matrix membranes. The zeolite Beta was used as a dispersed phase and its amount was varied between 10 to 40 wt.%. The effect of …
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Using a Mammalian Virus to Create Plants for Site-Specific Transgene Insertion
… Ti plasmid. The reporter gene, b-glucuronidase (GUS) was inserted proximal to AAVS1 in the plasmid for use in screening for the presence of T-DNA. In addition, it will serve as an indicator of the expression level expected for transgene inserted into AAVS1 by recombinant AAV. PCR amplification, …
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