Molecular characterization and promoter analysis of the maize cytosolic glyceraldehyde 3-phosphate dehydrogenase gene family

The response of maize (Zea mays L.) roots to oxygen deprivation involves an altered gene expression and protein synthesis. Maize cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been identified as one of the anaerobic polypeptides and mRNAs corresponding to a member of the gpc multi-gene family, gpc3, increase under anaerobiosis. This study extends further analysis of the gpc gene family, with specific emphasis on gpc4. Analysis of full length cDNA clones for GAPC2, GAPC3 and GAPC4 revealed that the deduced amino acid sequence of GAPC4 has 99.4% identity with the GAPC3 cDNA clone as compared to only 81% with either GAPC1 or GAPC2. Northern blot analysis revealed a maximum transcript accumulation 24 h after anaerobiosis and anoxic specific induced expression for gpc3 and gpc4.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Agronomy

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Manjunath, Sivalinganna

Contributors dc:contributor

• Sachs, Martin M.

• Agriculture, Agronomy
• Biology, Molecular
• Biology, Genetics

Statement dc:rights

• Copyright 1996 Manjunath, Sivalinganna

Language dc:language

eng

Identifier

AAI9624426
(UMI)AAI9624426

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/23079