Biochemical and immunocytochemical analysis of three novel microtubule-associated proteins in Drosophila melanogaster and the rat brain

Microtubules are essential organelles found in almost all eukaryotic cells. Some of their diverse functions include intracellular transport, cell division and maintenance of cell shape. Microtubules are composed of highly conserved proteins, the α- and β-tubulins. Many of the functions associated with microtubules are thought to be due to the proteins that associate with them and are collectively termed microtubule-associated proteins or MAPs. Microtubule proteins (MTP), isolated from Drosophila embryos by repetitive cycles of assembly and disassembly, have been analyzed during embryonic development. The yield of twice-cycled MTP increased an average of 15-fold from 8 to 12 hours of development. Analysis of MTP purified from 6 and 16 hours after fertilization reveal differences in MAP composition with the 6 hour MTP having an increased number of putative MAPs compared to the 16 hour fraction.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Srinivasan, Shaila

Contributors dc:contributor

• Karr, Timothy L.

• Biology, Neuroscience
• Biology, Cell
• Chemistry, Biochemistry

Statement dc:rights

• Copyright 1996 Srinivasan, Shaila

Language dc:language

eng

Identifier

AAI9625198
(UMI)AAI9625198

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/22689