An in vitro transcription termination system to analyze spinach chloroplast promoters

To determine whether chloroplast RNA polymerase would accurately terminate transcription in vitro, the 3$\sp\prime$ end of the rbcL gene as well as various factor-independent transcription terminators from E. coli were fused to the spinach chloroplast rbcL promoter. Transcription of the rbcL minigene did not result in production of the expected 265 nucleotide RNA. However, the spinach chloroplast RNA polymerase did terminate transcription with varying efficiency at the thra, rrnB, rrnC and gene 32 terminators. The most efficient transcription termination was observed for the thra terminator. For each of the prokaryotic terminators, the chloroplast enzyme ceased transcription at essentially the same position as the E. coli RNA polymerase.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Agronomy

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Chen, Liang-Jwu

Contributors dc:contributor

• Orozco, Emil M.

• Agriculture, Agronomy
• Biology, Molecular
• Biology, Botany

Statement dc:rights

• Copyright 1989 Chen, Liang-Jwu

Language dc:language

eng

Identifier

AAI9010825
(UMI)AAI9010825

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/22605