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Showing 1 to 20 of 86 for “"spinach"”.
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Characterization of the sex chromosomes in spinach
… Y chromosome (MSY) painstaking. Here we use YY spinach (Spinacia oleracea L.) to characterize the MSY and the X-specific region of the X chromosome. A screen of 395 accessions in the USDA germplasm collection found a single accession that segregates YY progeny. The YY genotype was verified by a …
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Analysis of tandemly and divergently oriented spinach chloroplast promoters
… that 3 out of 4 of the previously observed spinach atpB $5\sp\prime$ ends represent transcription initiation sites and not processing sites. A previously undetected transcript situated one codon 3$\sp\prime$ to the translation initiation site was also identified.
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The Risk Of Pythium Aphanidermatum In Hydroponic Baby-Leaf Spinach Production
… the production of hydroponically grown babyleaf spinach. This organism is so prevalent that even crops grown in fresh nutrient solution will often show signs of Pythium damage at harvest time. Previous studies have identified nutrient solution temperature as a key factor in determining the growth …
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Initial Fine Mapping of the Spinach Downy Mildew Resistance Locus RPF1
<p>Spinach is an important vegetable crop grown in many parts of the world (Correll et al. 2011). It has become increasingly popular due to its nutritional value as it is very high in antioxidants (Prior, 2003). Spinach production, however, is affected by many biotic stresses. Downy mildew, caused …
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The Relation of Environmental Conditions to The Storage Quality of Spinach
Made available in DSpace on 2014-12-05T19:06:49Z (GMT). No. of bitstreams: 1 6104347.pdf: 4547291 bytes, checksum: 662295ccde04620eac8c36a54be393c4 (MD5) Previous issue date: 1961
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An in vitro transcription termination system to analyze spinach chloroplast promoters
… terminators from E. coli were fused to the spinach chloroplast rbcL promoter. Transcription of the rbcL minigene did not result in production of the expected 265 nucleotide RNA. However, the spinach chloroplast RNA polymerase did terminate transcription with varying efficiency at the thra, …
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Creation and analysis of chimeras of spinach and tobacco Rubisco activase
… the activity of carbamylated enzyme. Previously spinach Rubisco activase had been cloned into the pPlex expression vector and the resulting polypeptide purified and characterized. I modified this clone to obtain protein truncated at the N and C terminus. Truncation at the N terminus had no effect …
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Pokeweed: mineral and oxalic acid content with a comparison to spinach
… iron, zinc, and oxalic acid. Since pokeweed and spinach are considered to be of similar eating quality, a comparison was made to like nutrient values of spinach. Pokeweed leaves produced the highest amount of phosphorus, zinc, and iron and the least amount of oxalic acid during early growth (May …
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The Mechanism of Chloride Activation of Oxygen Evolution in Spinach Photosystem Ii
… to directly monitor ${\rm Cl}\sp-$ binding in spinach. At low light intensity, the ${\rm Cl}\sp-$ activation curve (Hill activity vs. (${\rm Cl}\sp-$)) shows three intermediary plateaus in the concentration range 0.1-10 mM ${\rm Cl}\sp-,$ indicating kinetic cooperativity with respect to ${\rm …
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Site-directed mutagenesis of the ATP-binding site in spinach rubisco activase
Spinach rubisco activase has two isoforms derived from alternate splicing with the larger polypeptide containing an additional 4 kD C-terminal domain. Both isoforms, with molecular masses of 41 kD and 45 kD, were synthesized and purified from the transformed E. coli containing the cDNAs. A …
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Effects of electrostatic fields, ultrasonic vibrations and ultraviolet light on spinach seed
… of some electrically produced treatment on spinach seed. Seeds were treated between two 6-inch by 6-inch plates, 1-inch apart, at approximately 150 volts rms. Frequencies were 60, 500, and 900 cycles per second. Exposure times were 30, 60, and 90 seconds for each frequency. One sample was …
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Microbial Communities of Spinach at Various Stages of Plant Growth From Seed to Maturity
… plant development. The bacterial populations of spinach seed and leaves after germination were compared using DGGE, to assess bacterial community richness, and real-time PCR to compare the abundance of select phyla (total bacteria, <i>Actinobacteria, Bacteroidetes, Firmicutes, α-Proteobacteria …
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Thylakoid membrane from spinach - effect of processing on their function as appetite suppressing ingredient
Western diet contains a high proportion of palatable food, rich in refined carbohydrates and fat, that subsequently increases the risk of overconsumption and possibly leads to overweight and obesity. Therefore an approach to strengthen the inherent satiety for fat can be used to prevent …
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Genetic Resistance to the Downy Mildew Pathogen and Breeding towards Durable Disease Management in Spinach
<p>Spinach (Spinacia oleracea) is a self-pollinated, dioecious winter crop. Prevalent challenges to the production of spinach include disease pressure imposed by downy mildew, which is caused by Peronospora effusa (=P. farinosa f. sp. spinaciae [Pfs]). A total of 19 new races of P. effusa have …
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Regulation of Photosynthetic Carbon Metabolism in Isolated Spinach Chloroplasts Under Photorespiratory and Non-Photorespiratory Conditions
… on photosynthetic carbon metabolism in isolated spinach chloroplasts were examined. Under conditions of ambient O(,2), saturating HCO(,3)('-) and a range of phosphate concentrations (0.1-10 mM), PGA and DHAP (1-6 mM each) reduced ('14)CO(,2) fixation by 60% of the control rate when the ratio of …
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Genomic dissection of structural variations and evolution forces of the sex chromosomes in spinach (spinacia oleracea)
… sex chromosome evolution. We selected garden spinach, an annual leafy dioecious vegetable with a pair of nascent sex chromosomes to study sex chromosome evolution. Based on a theory of sex chromosome evolution, sex chromosomes evolved from autosomes by genetic mutations relate to the female …
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Identification and Optimization of the Antagonistic Potential of Native Spinach Microbiota towards Escherichia coli O157:H7
Leafy greens such as spinach have been the object of several recent food-borne pathogen outbreaks. The purpose of this study was to isolate bacteria spinach epiphytic bacteria that inhibit growth of E. coli O157:H7, which we describe as antagonism. The mechanism of antagonism was investigated and …
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Investigating the impact of three washing methods on antimicrobial efficacy, texture, and color of spinach and tomatoes
… and 1% citric acid) were investigated on spinach and tomatoes. The washing method parameters were optimized and the immediate impact of the three washing methods on bacterial reduction, texture, and color was evaluated. These were also measured over the shelf-life of the samples. 1% citric …
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Molecular Characterization of Spinach (Spinacia Oleracea) Microbial Community Structure and its Interaction With Escherichia coli O157:H7 in Modified Atmosphere Conditions
Leafy greens like lettuce and spinach are a common vehicle for foodborne illness in United States. It is unknown if native plant epiphytic bacteria may play a role in the establishment of enteric pathogens on leaf surfaces. The objective of this study was to characterize the bacterial communities …
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