Characterization and therapeutic alteration of the biliary excretion and enterohepatic cycling of zearalenone in sexually immature swine

The purposes of these studies were to characterize the biliary excretion and enterohepatic cycling (EHC) of zearlenone (ZEN) in young pigs and to therapeutically mimic the effect of bile removal in enhancing body clearance of ZEN. ($\sp3$H) ZEN was administered intravenously (IV), orally, and intravenously with bile removal (IVB) to female, 10-to 14-week-old pigs. The biological half life of total plasma radioactivity in IV and orally dosed pigs (86.6 hrs.) was much greater than that of IVB pigs (3.34 hrs.). Secondary peaks in plasma metabolite concentrations were seen during the terminal elimination phase in IV and oral animals and metabolites were still detectable at 48 hrs. postdosing. In IVB pigs, these peaks were absent, relative metabolite profiles were altered and ZEN and metabolites were no longer detectable after 16 hrs. Biliary recovery of radioactivity, principally as glucuronide conjugates was extensive (45.61 $\pm$ 4.73%) and significantly greater than that of fecal recovery in IV (6.56 $\pm$ 0.78%) or oral (21.74 $\pm$ 1.56%) pigs. Absorption of ZEN from the intestinal tract was estimated to be 80-85%. Intraduodenal administration of bile containing ($\sp3$H) ZEN and glucuronide metabolites resulted in recovery of 64.5 $\pm$ 4.89% of the dose in bile, 20.78 $\pm$ 3.94% in urine, and the presence of glucuronide conjugates of ZEN and α-zearalenol (ZEL) in portal and jugular plasma. Evidence for metabolism of ZEN by the intestinal mucosa was present.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Comparative Biosciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Biehl, Michael LeRoy

Contributors dc:contributor

• Buck, William B.

• Agriculture, Animal Pathology
• Biology, Veterinary Science

Statement dc:rights

• Copyright 1989 Biehl, Michael LeRoy

Language dc:language

eng

Identifier

AAI9010807
(UMI)AAI9010807

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/22563