University of Illinois at Urbana-Champaign
Thermodynamic and kinetic regulation of coupling factor
Abstract
dc:descriptionThe first part of this thesis focuses on measuring the energetic requirement for activating CF$\sb1$ in DTT-reduced and oxidized thylakoid membranes isolated from spinach, Spinacia oleracea L. The coupling factor complex H$\sp+$-ATPase has latent activity, i.e. it needs to be activated prior to being able to function catalytically. One aspect of activation is the presence of an electrochemical potential, where the magnitude of this potential is assessed as an energetic requirement for CF$\sb1$ activation. For this thesis the energetic requirement was assessed by monitoring the number of single-turnover flashes needed to initiate flash-induced ADP phosphorylation at varying $\Delta$G$\sb{\rm ATP}$ values.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Biology
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2011
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Grandoni, Patricia Ann
- Contributors dc:contributor
-
- Ort, Donald R.
Subjects
dc:subject × 1Rights
dc:rights- Statement dc:rights
-
- Copyright 1989 Grandoni, Patricia Ann
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
-
AAI8924826
(UMI)AAI8924826 - OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/22299