{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/22299"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/22299","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Thermodynamic and kinetic regulation of coupling factor","abstract":"The first part of this thesis focuses on measuring the energetic requirement for activating CF$\\sb1$ in DTT-reduced and oxidized thylakoid membranes isolated from spinach, Spinacia oleracea L. The coupling factor complex H$\\sp+$-ATPase has latent activity, i.e. it needs to be activated prior to being able to function catalytically. One aspect of activation is the presence of an electrochemical potential, where the magnitude of this potential is assessed as an energetic requirement for CF$\\sb1$ activation. For this thesis the energetic requirement was assessed by monitoring the number of single-turnover flashes needed to initiate flash-induced ADP phosphorylation at varying $\\Delta$G$\\sb{\\rm ATP}$ values.","abstract_html":"The first part of this thesis focuses on measuring the energetic requirement for activating CF$\\sb1$ in DTT-reduced and oxidized thylakoid membranes isolated from spinach, Spinacia oleracea L. The coupling factor complex H$\\sp+$-ATPase has latent activity, i.e. it needs to be activated prior to being able to function catalytically. One aspect of activation is the presence of an electrochemical potential, where the magnitude of this potential is assessed as an energetic requirement for CF$\\sb1$ activation. For this thesis the energetic requirement was assessed by monitoring the number of single-turnover flashes needed to initiate flash-induced ADP phosphorylation at varying $\\Delta$G$\\sb{\\rm ATP}$ values.","abstract_has_math":true,"creators":["Grandoni, Patricia Ann"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Ort, Donald R."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:35:27Z","date_published":"2011-05-07T13:35:27Z","updated_at":"2026-07-22T22:25:19Z","subjects":["Biology, Plant Physiology"],"languages":["eng"],"rights":["Copyright 1989 Grandoni, Patricia Ann"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI8924826","(UMI)AAI8924826"],"render_values":[{"text":"AAI8924826","href":null,"code":true},{"text":"(UMI)AAI8924826","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/22299","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Ort, Donald R."]},{"key":"dc:creator","label":"Author","values":["Grandoni, Patricia Ann"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:35:27Z","10000-01-01","1989"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Plant Physiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1989 Grandoni, Patricia Ann"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI8924826","(UMI)AAI8924826","http://hdl.handle.net/2142/22299"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The first part of this thesis focuses on measuring the energetic requirement for activating CF$\\sb1$ in DTT-reduced and oxidized thylakoid membranes isolated from spinach, Spinacia oleracea L. The coupling factor complex H$\\sp+$-ATPase has latent activity, i.e. it needs to be activated prior to being able to function catalytically. One aspect of activation is the presence of an electrochemical potential, where the magnitude of this potential is assessed as an energetic requirement for CF$\\sb1$ activation. For this thesis the energetic requirement was assessed by monitoring the number of single-turnover flashes needed to initiate flash-induced ADP phosphorylation at varying $\\Delta$G$\\sb{\\rm ATP}$ values.","The second part of this thesis focuses on investigating the decline in flash yield (the amount of ATP synthesized/flash), seen with both DTT-reduced and oxidized thylakoid membranes, when flash-induced ATP synthesis is measured at elevated $\\Delta$G$\\sb{\\rm ATP}$ values. The data from these experiments show that coupling factor reversibility is not responsible for the drop in flash yield. The presence of the electrochemical potential promotes net ATP synthesis and likewise limits the amount of ATP hydrolysis. The regulatory effect of the electrochemical potential is demonstrated when a partial relaxation of the electrochemical potential enhances hydrolysis. These data suggest that a minimal electrochemical potential is required to activate the complex and this potential must be maintained to maintain the coupling factor in an activated conformation. (Abstract shortened with permission of author.)","Made available in DSpace on 2011-05-07T13:35:27Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 8924826.pdf: 4936619 bytes, checksum: b289790e9c2380c7c74c30498533d2a2 (MD5) Previous issue date: 1989","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:56:40Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:26:31-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Thermodynamic and kinetic regulation of coupling factor"]}]}],"canonical_facts":{"dc:contributor":["Ort, Donald R."],"dc:creator":["Grandoni, Patricia Ann"],"dc:date":["2011-05-07T13:35:27Z","10000-01-01","1989"],"dc:description":["The first part of this thesis focuses on measuring the energetic requirement for activating CF$\\sb1$ in DTT-reduced and oxidized thylakoid membranes isolated from spinach, Spinacia oleracea L. The coupling factor complex H$\\sp+$-ATPase has latent activity, i.e. it needs to be activated prior to being able to function catalytically. One aspect of activation is the presence of an electrochemical potential, where the magnitude of this potential is assessed as an energetic requirement for CF$\\sb1$ activation. For this thesis the energetic requirement was assessed by monitoring the number of single-turnover flashes needed to initiate flash-induced ADP phosphorylation at varying $\\Delta$G$\\sb{\\rm ATP}$ values.","The second part of this thesis focuses on investigating the decline in flash yield (the amount of ATP synthesized/flash), seen with both DTT-reduced and oxidized thylakoid membranes, when flash-induced ATP synthesis is measured at elevated $\\Delta$G$\\sb{\\rm ATP}$ values. The data from these experiments show that coupling factor reversibility is not responsible for the drop in flash yield. The presence of the electrochemical potential promotes net ATP synthesis and likewise limits the amount of ATP hydrolysis. The regulatory effect of the electrochemical potential is demonstrated when a partial relaxation of the electrochemical potential enhances hydrolysis. These data suggest that a minimal electrochemical potential is required to activate the complex and this potential must be maintained to maintain the coupling factor in an activated conformation. (Abstract shortened with permission of author.)","Made available in DSpace on 2011-05-07T13:35:27Z (GMT). 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