High-pressure NMR studies of model membranes

The effects of pressure, up to 5 kbar, on phospholipid vesicles were measured using high pressure NMR techniques. Specifically, two sets of experiments were performed. First, sonicated vesicles of 1-palmitoyl-2-oleyl-3-sn-phosphatidyl-choline at 28$\sp\circ$C were studied using two-dimensional phase sensitive NOESY as an indicator of cross-relaxation in the bilayer. Results indicated a dramatic buildup of cross-peak intensity with pressure. A qualitative explanation of the results, using a well-known model of cross-relaxation in the lipid bilayer, was that high pressure causes an increase in the order parameter throughout the acyl chains, directly increasing the measured cross-relaxation rate and steady-state value of the NOE.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Driscoll, Daniel Abram

Contributors dc:contributor

• Jonas, Ana

• Chemistry, Biochemistry
• Chemistry, Physical
• Biophysics, General

Statement dc:rights

• Copyright 1990 Driscoll, Daniel Abram

Language dc:language

eng

Identifier

AAI9114227
(UMI)AAI9114227

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/22239