{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/22239"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/22239","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"High-pressure NMR studies of model membranes","abstract":"The effects of pressure, up to 5 kbar, on phospholipid vesicles were measured using high pressure NMR techniques. Specifically, two sets of experiments were performed. First, sonicated vesicles of 1-palmitoyl-2-oleyl-3-sn-phosphatidyl-choline at 28$\\sp\\circ$C were studied using two-dimensional phase sensitive NOESY as an indicator of cross-relaxation in the bilayer. Results indicated a dramatic buildup of cross-peak intensity with pressure. A qualitative explanation of the results, using a well-known model of cross-relaxation in the lipid bilayer, was that high pressure causes an increase in the order parameter throughout the acyl chains, directly increasing the measured cross-relaxation rate and steady-state value of the NOE.","abstract_html":"The effects of pressure, up to 5 kbar, on phospholipid vesicles were measured using high pressure NMR techniques. Specifically, two sets of experiments were performed. First, sonicated vesicles of 1-palmitoyl-2-oleyl-3-sn-phosphatidyl-choline at 28$\\sp\\circ$C were studied using two-dimensional phase sensitive NOESY as an indicator of cross-relaxation in the bilayer. Results indicated a dramatic buildup of cross-peak intensity with pressure. A qualitative explanation of the results, using a well-known model of cross-relaxation in the lipid bilayer, was that high pressure causes an increase in the order parameter throughout the acyl chains, directly increasing the measured cross-relaxation rate and steady-state value of the NOE.","abstract_has_math":true,"creators":["Driscoll, Daniel Abram"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["Jonas, Ana"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:33:31Z","date_published":"2011-05-07T13:33:31Z","updated_at":"2026-07-22T22:25:19Z","subjects":["Chemistry, Biochemistry","Chemistry, Physical","Biophysics, General"],"languages":["eng"],"rights":["Copyright 1990 Driscoll, Daniel Abram"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9114227","(UMI)AAI9114227"],"render_values":[{"text":"AAI9114227","href":null,"code":true},{"text":"(UMI)AAI9114227","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/22239","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Jonas, Ana"]},{"key":"dc:creator","label":"Author","values":["Driscoll, Daniel Abram"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:33:31Z","10000-01-01","1990"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Biochemistry","Chemistry, Physical","Biophysics, General"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1990 Driscoll, Daniel Abram"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9114227","(UMI)AAI9114227","http://hdl.handle.net/2142/22239"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The effects of pressure, up to 5 kbar, on phospholipid vesicles were measured using high pressure NMR techniques. Specifically, two sets of experiments were performed. First, sonicated vesicles of 1-palmitoyl-2-oleyl-3-sn-phosphatidyl-choline at 28$\\sp\\circ$C were studied using two-dimensional phase sensitive NOESY as an indicator of cross-relaxation in the bilayer. Results indicated a dramatic buildup of cross-peak intensity with pressure. A qualitative explanation of the results, using a well-known model of cross-relaxation in the lipid bilayer, was that high pressure causes an increase in the order parameter throughout the acyl chains, directly increasing the measured cross-relaxation rate and steady-state value of the NOE.","The second set of experiments were done on multilamellar vesicles of tail-perdeuterated dipalmitoyl phosphatidylcholine, using high pressure deuterium MMR as a probe of the order in this system as pressure was varied at several different temperatures. Measurements were taken both in the liquid-crystalline and gel states of pure lipid bilayers. Effects and reversal of action of the local anesthetic tetracaine were studied. The results showed that the order parameters of all segments of the chain were increased with pressure. The addition of tetracaine disordered the chain, and this effect was shown to be reversed by pressure by both changes in lineshape and the measured order parameters. The application of pressure in the gel state had the same effect as lowering the temperature, in that the gel state lineshape slowly changed from a motionally averaged to a rigid lattice type spectrum as pressure was increased. At very high pressures, even the methyl portion of the spectrum indicated no motion on the MMR timescale. A phase which corresponded to an interdigitated gel was observed, and a general phase diagram constructed.","Made available in DSpace on 2011-05-07T13:33:31Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9114227.pdf: 4781573 bytes, checksum: e39fdd82f62a6bf35df2a0f8e7355ae6 (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:56:16Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:26:17-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["High-pressure NMR studies of model membranes"]}]}],"canonical_facts":{"dc:contributor":["Jonas, Ana"],"dc:creator":["Driscoll, Daniel Abram"],"dc:date":["2011-05-07T13:33:31Z","10000-01-01","1990"],"dc:description":["The effects of pressure, up to 5 kbar, on phospholipid vesicles were measured using high pressure NMR techniques. Specifically, two sets of experiments were performed. First, sonicated vesicles of 1-palmitoyl-2-oleyl-3-sn-phosphatidyl-choline at 28$\\sp\\circ$C were studied using two-dimensional phase sensitive NOESY as an indicator of cross-relaxation in the bilayer. Results indicated a dramatic buildup of cross-peak intensity with pressure. A qualitative explanation of the results, using a well-known model of cross-relaxation in the lipid bilayer, was that high pressure causes an increase in the order parameter throughout the acyl chains, directly increasing the measured cross-relaxation rate and steady-state value of the NOE.","The second set of experiments were done on multilamellar vesicles of tail-perdeuterated dipalmitoyl phosphatidylcholine, using high pressure deuterium MMR as a probe of the order in this system as pressure was varied at several different temperatures. Measurements were taken both in the liquid-crystalline and gel states of pure lipid bilayers. Effects and reversal of action of the local anesthetic tetracaine were studied. The results showed that the order parameters of all segments of the chain were increased with pressure. The addition of tetracaine disordered the chain, and this effect was shown to be reversed by pressure by both changes in lineshape and the measured order parameters. The application of pressure in the gel state had the same effect as lowering the temperature, in that the gel state lineshape slowly changed from a motionally averaged to a rigid lattice type spectrum as pressure was increased. At very high pressures, even the methyl portion of the spectrum indicated no motion on the MMR timescale. A phase which corresponded to an interdigitated gel was observed, and a general phase diagram constructed.","Made available in DSpace on 2011-05-07T13:33:31Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9114227.pdf: 4781573 bytes, checksum: e39fdd82f62a6bf35df2a0f8e7355ae6 (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:56:16Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:26:17-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9114227","(UMI)AAI9114227","http://hdl.handle.net/2142/22239"],"dc:language":["eng"],"dc:rights":["Copyright 1990 Driscoll, Daniel Abram"],"dc:subject":["Chemistry, Biochemistry","Chemistry, Physical","Biophysics, General"],"dc:title":["High-pressure NMR studies of model membranes"],"dc:type":["text"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:19Z"}