The mechanism of component recognition of bipartite geminiviruses: Genetic studies of the replication of squash leaf curl virus, and, Genetic and functional studies of squash leaf curl virus coat protein AR1

Both genomic components of bipartite geminiviruses encode functions essential for viral infection: the A component replicates the viral genome and the B component systemically spreads the virus throughout the host. Thus, correct recognition between the two components is required to ensure a constant co-presence of the two viral genomic components during viral infection. In this study, using two closely related yet sufficiently different strains of squash leaf curl virus (SqLCV) as a model system, I will show that: (1) viral replication is an important step in component recognition of SqLCV infection; (2) the sequence between the directly repeated motif and the stem-loop structure of SqLCV replication origin is involved in component recognition and contains specific sequence element for efficient viral replication; (3) replication protein AL1 interacts with the sequence between the directly repeated motif and the stem-loop structure to balance replication of the two viral components; (4) balanced replication of the two viral components constitutes the molecular basis of component recognition of SqLCV.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Qin, Shenwei

Contributors dc:contributor

• Lazarowitz, Sondra G.

• Biology, Molecular
• Biology, Microbiology

Statement dc:rights

• Copyright 1996 Qin, Shenwei

Language dc:language

eng

Identifier

(UMI)AAI9712410
9780591200393
AAI9712410

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/22218