Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"replication origin"”.
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Changing protein-DNA interactions promote ORC binding site exchange during replication origin licensing
DNA replication is a fundamental process that ensures the faithful duplication of genetic material in all living organisms. In eukaryotic cells, DNA replication proceeds bidirectionally from origins of replication. At the heart of this process is the eukaryotic replicative helicase, Mcm2-7, which …
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The genome-wide analysis of the specification and activation of human chromosomal DNA replication origin sites
Background: Human DNA replication is a critical cellular process that initiates at tens of thousands of DNA replication origins. Next-generation sequencing (NGS) enabled the elucidation of some characteristics that define human replication origins. Initiation-site sequencing (iniSeq) utilises the …
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Peak Mitotic Cyclin Permits Mitotic Exit
<p>In eukaryotes, DNA replication, mitosis, and cytokinesis are all regulated by Cyclin-dependent kinase (Cdk). Cyclin/Cdk complexes promote replication origin firing and mitotic entry, and conversely, inhibit pre-replication origin loading and exit from mitosis. Cyclin synthesis and degradation, …
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The mechanism of component recognition of bipartite geminiviruses: Genetic studies of the replication of squash leaf curl virus, and, Genetic and functional studies of squash leaf curl virus coat protein AR1
… as a model system, I will show that: (1) viral replication is an important step in component recognition of SqLCV infection; (2) the sequence between the directly repeated motif and the stem-loop structure of SqLCV replication origin is involved in component recognition and contains specific …
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Construction and Use of a Transposon for Identification of Essential Genes in Mycobacteria
… onto a vector with a temperature sensitive replication origin for more efficient mutagenesis of mycobacteria. In a preliminary genetic screen using the model organism Mycobacterium smegmatis, 13 mutants with ATc-dependent growth were identified. Identification of the insertion sites by …
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Probabilistic Modelling of Replication Fidelity in Eukaryotic Genomes
Eukaryotic DNA replication is composed of a complex array of molecular biological activities compounded by the pressure for faithful replication in order to maintain genetic and genomic integrity. The constraints governing DNA replication biology is of fundamental importance to understand the …
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Genome-wide Footprinting Uncovers Epigenetic Regulatory Paradigms by Revealing the Chromatin Occupancy Landscape
… and plasticity to modify transcription and replication programs, yielding a myriad of differentiated cell types and survival mechanisms to adverse environmental conditions. As these genomic processes require precise localization of DNA-binding factors, their dynamic temporal and spatial …
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Modeling Multi-factor Binding of the Genome
… proteins and protein complexes, such as the origin recognition complex (ORC). DBFs compete with one another for binding along the genome, yet many current models of genome binding do not consider different types of DBFs together simultaneously. Additionally, binding is a stochastic process …
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ONCOGENE-INDUCED ALTERED DNA REPLICATION DYNAMICS
… By performing a whole genome analysis of DNA replication dynamics occurring upon oncogene activation, I discovered that oncogene activation alters DNA replication by increasing replication fork speed and fork stalling, while decreasing the frequency of replication initiation. This is …
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Assay for Rifampicin Resistant M13 RF Synthesis
… rapid assay which can detect the function of the origin of 0X174 complementary strand DNA in Ml3 by virtue of its ability to initiate the synthesis of M13 complementary strands in the presence of the antibiotic rifampicin. A segment of DNA carrying the sequence recognized by the replication …
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Establishing DNA combing to investigate DNA replication stress in vitro
… replicate their DNA utilizing multiple DNA replication sites known as origins of replication. DNA is replicated to provide each daughter cell a complete copy of the genome. Replication proceeds bi-directionally from a minority of potential origins licensed for replication by a variety of …
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Selection Dynamics in the Germinal Center During Antibody Affinity Maturation
… is regulated by increasing the speed of DNA replication forks, while maintaining the dynamics of replication origin activation throughout the genome. Thus, T cells mediate affinity-based selection in the GC by inducing selected cells to reside longer in the DZ where they undergo accelerated …
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Clarifying the Role of the CST Complex in DNA Replication and Repair
… additional genome-wide roles in regulating DNA replication and repair including helping facilitate replication re-start at stalled forks, activating checkpoint signaling at double-strand breaks, and promoting replication origin firing. Proper and efficient repair of DNA is critical in order to …
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DNA-Protein Interactions in f1 DNA Replication
… of two DNA-binding proteins with the plus strand replication origin of bacteriophage f1. The origin has a bipartite structure consisting of a required core origin region and an adjacent A+T- rich enhancer sequence that potentiates replication approximately 100-fold. The core origin binds the …
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The role of recombinational repair in the mechanism of killing during thymine starvation in Escherichia coli
… lesions. I first characterized the extent of DNA replication, stability and damage during TLD using physical methods, such as pulse-field gel electrophoresis to measure levels of double strand breaks. I next investigated the role of major DNA repair and recombinational repair pathways by studying …
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Functions of RTF2 in DNA Replication and Checkpoint Control
… has uncovered one such dynamic process at replication forks experiencing replication stress, by which proteasome shuttle proteins DNA damage inducible 1 and DNA damage inducible 2 (DDI1/2) regulate the level of replication termination factor 2 (RTF2) at the replisome. When RTF2 is retained …
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Origins of non-coding Y RNA family and its role in DNA replication
… RNAs that are required for chromosomal DNA replication initiation in vertebrates. Specifically, Y RNAs play an essential role in the establishment of new DNA replication forks during G1 to S phase transition. However, the mechanism by which Y RNAs contribute to initiation of DNA replication, …