Studies of sequence recognition by the EcoRI restriction/modification system of Escherichia coli

Sequence recognition by the EcoRI restriction/modification system of Escherichia coli is currently the subject of intense study as a model of the general phenomenon of target location by nucleotide sequence-specific enzymes and binding proteins. Models have proposed a variety of means for site location, including formation of hydrogen bond matrices between protein and DNA and ionic contacts between the ribose-phosphate backbone and basic amino acids residues on the protein. We have examined DNA-protein interactions involved in the EcoRI recognition process by using defined oligonucleotide duplex substrates with various alterations inside and outside the recognition site. This model system consists of an endonuclease that cleaves the DNA sequence, GAATTC, between the guanine and adenine residues and a methyltransferase that methylates the 6-amino group of the central adenine residue. The advantages of this model system include the ability to study recognition of the same substrates by two different enzymes and the existence of a large context of information to direct and supplement the investigation.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Van Cleve, Mark David

• Chemistry, Biochemistry

Statement dc:rights

• Copyright 1990 Van Cleve, Mark David

Language dc:language

eng

Identifier

AAI9026341
(UMI)AAI9026341

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/22122