Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 32 for “"Restriction-Modification"”.
-
A Novel Method to Detect Bacterial Restriction-Modification (R-M) Systems
… DNA sequences that code for previously unknown restriction endonucleases and their corresponding methyltransferases. These findings show that numerous restriction enzymes abundant in bacteria have yet to be discovered. Traditionally, restriction enzymes have been discovered by the classical …
-
The Expression of DNA Restriction-Modification Genes of ESCHERICHIA COLI K12
<p>To facilitate the study of the expression of hsd genes in Escherichia coli K, hsdK::MudX (amp crop lac) operon fusion mutants have successfully been constructed and characterized for the first time. The results indicate that Promoters 1 and 2 for hsdK genes appear to be relatively weak, as …
-
Non-parental-type Recombinants in Crosses between Different Restriction-Modification Systems in SALMONELLA
<p>Many new restriction-modification (R-M) system which are closely linked to <em>serB</em> and allelic to the <em>hsd</em><sub>SB</sub> system of <em>S. typimurium</em> occur in different <em>Salmonella</em> serotypes. In PI transduction studies with these systems, recombinants were isolated which …
-
Studies of sequence recognition by the EcoRI restriction/modification system of Escherichia coli
Sequence recognition by the EcoRI restriction/modification system of Escherichia coli is currently the subject of intense study as a model of the general phenomenon of target location by nucleotide sequence-specific enzymes and binding proteins. Models have proposed a variety of means for site …
-
Restriction-Modification and CRISPR-Cas Systems: Cooperation Between Innate and Adaptive Immunity in Prokaryotes
… or simply phages) that can infect and kill them. Restriction-modification (RM) systems represent one such strategy. Generally, these systems provide defense by coordinating the activities of two distinct enzymes: a restriction endonuclease and a methyltransferase. Both enzymes recognize the same …
-
Deoxyribonucleic acid restriction/modification systems and gene transfer strategies in Ruminococcus albus 8 and Ruminococcus flavefaciens FD-1
… FD-1. The limited degradation of DNA by restriction enzymes and confirmation of plasmid uptake were given priority, although the consequences of incompatible plasmid replicons and failure in the expression of selectable markers cannot be overlooked. Fluorescent labelled dextrans were used …
-
Molecular Cloning and Analysis of the Modification Genes of the KpnBl Restriction- Modification System of Klebsiella pneumoniae Strain GM236
<p>The <em>KpnB</em>l restriction-modification (R-M) system has been recognized for some time in <em>Klebsiella pneumoniae</em> strain GM236. Previously the restriction subunit (<em>hsdR</em><sub>kpmBI</sub>) was cloned and sequenced, based on the sequence infonnation the <em>Kpn</em>Bl RM system …
-
Investigating Epigenetics as a Mechanism to Regulate Bacterial Heterologous Expression
… transcription. Epigenetic methylation as part of restriction-modification systems is frequently considered a barrier in synthetic biology since it can prevent the introduction of heterologous genes to new host organisms. Due to this, many studies that construct bacterial hosts for use in synthetic …
-
Genetic studies of Streptomyces cattleya and Streptomyces olivaceus
… lividans TC10 indicated the absence of restriction-modification systems which affect VCll infectivity of these Streptomyces strains.
-
Mechanistic studies of Fusobacterium genetic and defense systems
… editing. Accordingly, we first investigated restriction-modification systems with a focus on the role of DNA methyltransferases and endonucleases in host defense and genetic recalcitrance in several strains of Fusobacterium through bioinformatic and biochemical approaches. Altogether, over 15 …
-
A Fast Assay of Bacteria Cell Permeability for Genetic Transformation
… from confounding factors such as restriction-modification systems. As a result, it provides a more accurate qualitative and quantitative assessment of bacterial membrane disruption. This approach enables same-day evaluation of electroporation conditions regardless of bacterial …
-
Genome sequence of the hyperinvasive Campylobacter jejuni strains
… lipooligosaccharide, flagellar biosynthesis and restriction modification regions were the most diverse. The hierarchical clustering based on comparative genomic hybridization (CGH) did not group together the hyperinvasive C. jejuni as a single group and these strains possessed different MLST …
-
Niche-adaptive evolution in Campylobacter jejuni
… lineage specific content, in the form of Restriction-Modification (R-M) system associated genes, and to have undergone degredation of certain loci. The ST403CC isolates also exhibited a distinct pattern of reduced genomic recombination compared to non-ST403CC C. jejuni, with evidence of …
-
Comparative and Functional Genomic Studies of Histophilus somni (Haemophilus somnus)
… putative genes that encode proteases, restriction-modification enzymes, hemagglutinins, glycosyltransferases, kinases, helicases, and adhesins have been identified in H. somni. Most of the H. somni strain-specific genes were found to be associated with prophage-like sequences, plasmids, …
-
Towards Novel Methods of Mutagenesis for Histophilus somni
… allelic exchange is difficult due to its tight restriction modification system. Mutagenesis by natural transformation in Haemophilus influenzae is well established and may be enhanced by the presence of uptake signal sequences (USS) within the genome. We hypothesized that natural transformation …
-
Prevotella Phylogeny: Genomic And Molecular Insights Into The Role Of The Human Commensal Prevotella In Cystic Fibrosis
… have high recombination rates but also complex restriction-modification systems. Our work highlights the incredible genomic diversity within some of the oropharyngeal commensal Prevotella, indicating that the key to drawing meaningful associations of Prevotella with health and disease is by …
-
The evolutionary ecology of methylation-dependent bacterial defense systems
… be defense class specific, we focus initially on restriction modification systems, which are both ubiquitous and confer broad resistance, making them highly important in host-MGE coevolution. Firstly, using flow cytometry and high-throughput competition and coevolution experiments we demonstrate …
-
Mechanisms of phage detection by bacterial innate immune proteins
… protect themselves from phage infection, such as restriction modification, CRISPR-Cas, and abortive infection (Abi) systems. Because Abi systems function through killing infected cells to protect the bacterial population, they must stay inactive prior to infection, but rapidly detect phages and …
-
The development of Sinorhizobium meliloti and Deinococcus radiodurans as chassis for synthetic biology applications
… through the sequential genetic knockout of four restriction-modification systems. The tools and strains developed in my thesis will add to the growing genetic toolkits of S. meliloti and D. radiodurans. The establishment of S. meliloti as an alternative chassis for interkingdom DNA transfer will …
Page 1 of 2