The Expression of DNA Restriction-Modification Genes of ESCHERICHIA COLI K12

<p>To facilitate the study of the expression of hsd genes in Escherichia coli K, hsdK::MudX (amp crop lac) operon fusion mutants have successfully been constructed and characterized for the first time. The results indicate that Promoters 1 and 2 for hsdK genes appear to be relatively weak, as reflected by about 50 units of beta-gal activity. Both promoters were active in all the conditions tested except during Beta-gal activity was higher in aerobic than in anaerobic growth; at 30°C and 37°C than at 42°C; and in L-broth early log phase of the culture. than in minimal media. Potential future uses of these fusion mutants are discussed.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Microbiology

Year

1986

Author dc:creator

• Prakash, Ainu

Contributors dc:contributor

• Jun-Ichi Ryu
• Anthony J. Zuccarelli
• Eric A. Goulbourne, Jr.

• Microbiology
• DNA Restriction Enzymes -- biosynthesis; Escherichia coli -- analysis.

Statement dc:rights

• This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Language dc:language

English

Repository record dc:identifier

https://scholarsrepository.llu.edu/etd/686

OAI identifier oai:identifier

oai:scholarsrepository.llu.edu:etd-1814