To facilitate the study of the expression of hsd genes in Escherichia coli K, hsdK::MudX (amp crop lac) operon fusion mutants have successfully been constructed and characterized for the first time. The results indicate that Promoters 1 and 2 for hsdK genes appear to be relatively weak, as reflected by about 50 units of beta-gal activity. Both promoters were active in all the conditions tested except during Beta-gal activity was higher in aerobic than in anaerobic growth; at 30°C and 37°C than at 42°C; and in L-broth early log phase of the culture. than in minimal media. Potential future uses of these fusion mutants are discussed.
","abstract_html":"<p>To facilitate the study of the expression of hsd genes in Escherichia coli K, hsdK::MudX (amp crop lac) operon fusion mutants have successfully been constructed and characterized for the first time. The results indicate that Promoters 1 and 2 for hsdK genes appear to be relatively weak, as reflected by about 50 units of beta-gal activity. Both promoters were active in all the conditions tested except during Beta-gal activity was higher in aerobic than in anaerobic growth; at 30°C and 37°C than at 42°C; and in L-broth early log phase of the culture. than in minimal media. Potential future uses of these fusion mutants are discussed.</p>","abstract_has_math":false,"creators":["Prakash, Ainu"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Thesis","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Jun-Ichi Ryu","Anthony J. Zuccarelli","Eric A. Goulbourne, Jr."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1986,"date_issued":"1986-09-01T07:00:00Z","date_published":"1986-09-01T07:00:00Z","updated_at":"2026-07-24T02:53:08Z","subjects":["Microbiology","DNA Restriction Enzymes -- biosynthesis; Escherichia coli -- analysis."],"languages":["English"],"rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarsrepository.llu.edu/etd/686","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Jun-Ichi Ryu","Anthony J. Zuccarelli","Eric A. Goulbourne, Jr."]},{"key":"dc:creator","label":"Author","values":["Prakash, Ainu"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Microbiology","DNA Restriction Enzymes -- biosynthesis; Escherichia coli -- analysis."]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarsrepository.llu.edu/etd/686"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["To facilitate the study of the expression of hsd genes in Escherichia coli K, hsdK::MudX (amp crop lac) operon fusion mutants have successfully been constructed and characterized for the first time. The results indicate that Promoters 1 and 2 for hsdK genes appear to be relatively weak, as reflected by about 50 units of beta-gal activity. Both promoters were active in all the conditions tested except during Beta-gal activity was higher in aerobic than in anaerobic growth; at 30°C and 37°C than at 42°C; and in L-broth early log phase of the culture. than in minimal media. Potential future uses of these fusion mutants are discussed.
"]},{"key":"dc:title","label":"Title","values":["The Expression of DNA Restriction-Modification Genes of ESCHERICHIA COLI K12"]}]}],"canonical_facts":{"dc:contributor":["Jun-Ichi Ryu","Anthony J. Zuccarelli","Eric A. Goulbourne, Jr."],"dc:creator":["Prakash, Ainu"],"dc:description.abstract":["To facilitate the study of the expression of hsd genes in Escherichia coli K, hsdK::MudX (amp crop lac) operon fusion mutants have successfully been constructed and characterized for the first time. The results indicate that Promoters 1 and 2 for hsdK genes appear to be relatively weak, as reflected by about 50 units of beta-gal activity. Both promoters were active in all the conditions tested except during Beta-gal activity was higher in aerobic than in anaerobic growth; at 30°C and 37°C than at 42°C; and in L-broth early log phase of the culture. than in minimal media. Potential future uses of these fusion mutants are discussed.
"],"dc:identifier":["https://scholarsrepository.llu.edu/etd/686"],"dc:language":["English"],"dc:rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"dc:subject":["Microbiology","DNA Restriction Enzymes -- biosynthesis; Escherichia coli -- analysis."],"dc:title":["The Expression of DNA Restriction-Modification Genes of ESCHERICHIA COLI K12"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T02:53:08Z"}