Covalent post-translational modification of proteins in Escherichia coli: Genetic and biochemical studies of biotin and lipoic acid biosynthesis and function

I have identified four distinct classes of mutants involved in either lipoic acid biosynthesis or the covalent attachment of lipoic acid to proteins in E. coli. Two of these classes of mutants were isolated as Tn1000dKn insertion mutants and both classes map to min 14.5 on the E. coli chromosome. The other two classes of mutants were isolated as spontaneous selenolipoic acid resistant (slr) mutants. Selenolipoic acid was shown to be a potent inhibitor of wild type E. coli.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Reed, Kelynne Elizabeth

Contributors dc:contributor

• Cronan, John E.

• Biology, Microbiology

Statement dc:rights

• Copyright 1992 Reed, Kelynne Elizabeth

Language dc:language

eng

Identifier

AAI9305663
(UMI)AAI9305663

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/22104