Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 281 for “"Post-translational Modification"”.
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Post-Translational Modification With Lipoic Acid in Escherichia Coli
Lipoic acid is a cofactor essential for the function of key metabolic pathways in eukaryotes and most prokaryotes. Physiological function of lipoic acid is dependent on covalent attachment of the cofactor to highly specific lysine residues of enzymes complex subunits. E. coli encodes two distinct …
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Algorithms to Detect Motifs and Predict Post-Translational Modification Sites
Post-translational modifications (PTMs) are an important step in the formation of a mature protein. While much is known about some PTMs such as Phosphorylation, there are other PTMs such as Cysteine S-sulfenylation that are currently being researched and analyzed. For this project, an algorithm, …
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Towards targeted post-translational modification of endogenous proteins in complex environments
… amino acid side chains, brought about by co- and post-translational modifications (PTMs). Depending on the roles of the modified amino acids and the nature of the chemical changes, PTMs can have tremendous influence on protein function. This phenomenon can be exploited pharmacologically, as …
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Exploring the Dynamics of Mechanosensitive Channels Utilizing a Post Translational Modification Approach
… We investigate this hypothesis by using a post-translational modification approach to determine how changing hydrophobicity or charge at specific locations influences gating. Briefly, we used MscL channels with specific residues in the linker between the second transmembrane region (TM2) …
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Biochemical characterization of enzymes involved in the post-translational modification of lantibiotics
The student, Manuel Ortega, accepted the attached license on 2015-11-05 at 09:42.
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Functional significance of Hsp70 post-translational modification in prion propagation and cellular function
… regulation of this important chaperone at the post-translational level. Recent global proteomic studies have demonstrated that in vivo Hsp70 is phosphorylated. Using a simplified yeast system this study systematically assessed a variety of non-phosphorylateable and phosphomimetic Hsp70 mutants …
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Post-Translational Modification of Proteins via Ambient Air Pollutants and Endogenous Reactive Species
… and reactive nitrogen species (RNS) to form post-translational modifications (PTMs), which can affect protein structure and function. The formation of 3-nitrotyrosine (NTyr) and dityrosine (DiTyr) upon reaction of proteins with ROS/RNS are two common PTMs studied due to their stability and …
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Histone Post-translational Modification Dysregulation Contributes to Toxicity in Amyotrophic Lateral Sclerosis Proteinopathy Models
… unrelated to changes in DNA sequence. Histone modifications, a main epigenetic mechanism, occur in many amino acid residues and include phosphorylation, acetylation, methylation as well as other chemical moieties. Recent evidence points to a possible role for epigenetic mechanisms in the …
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Engineering of the microviridin post-translational modification enzymes for the production of synthetic protease inhibitors
… products. The ribosomally synthesized and post-translationally modified peptides (RiPPs) are a group of natural products generated by the action of post-translationally modifying enzymes on precursor peptides translated from mRNA by ribosomes. The great substrate promiscuity exhibited by …
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Post-translational modification of proteins secreted by Pseudomonas aeruginosa and its role in bacterial physiology
… at the transcriptional level, but also through post-translational modification (PTM). By understanding the nature of PTM in extracellular proteins and how these may affect protein function and stability, a future platform for alternative anti-virulence interventions can be developed. In this …
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Investigating the Post-Translational Modification of Notch1 Intracellular Domain: From Proteolytic Cleavage to Mass Spectrometric Profiling
… has been widely associated with cancer. Thus, post-translational modifications (PTM) further fine-tune Notch activity by modulating its trafficking, stability, and transcriptional dynamics. This thesis investigates the post-translational regulation of the Notch1 intracellular domain (N1ICD), …
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Biochemical investigation of phosphodiesterase type IV post-translational modification, cellular localisation and interaction with associated binding proteins
… scaffold PKA regulatory subunits. In this study post-translational-modification of PDE4 isoforms is investigated. SUMOylation is a relatively newly identified post-translational modification that is known to regulate the structure and function of its substrates. PDE4 isoforms of the PDE4A and 4D …
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The isolation and characterisation of MHC-presented peptides from CML-derived cell-lines, with a focus on post-translational modification
Phosphorylation is a key regulator of protein function and activity, and aberrant kinase activity is implicated in a wide range of malignancies, of which the bcr:abl fusion kinase found in chronic myeloid leukaemia is a classic example. As phosphopeptides are known to be presented by both the MHC …
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Disordered Protein Aggregates Are Linked to Changes in the Histone Post-Translational Modification Landscape in Disease and Non-Disease Models
<p>Proper protein folding is a delicate balance that is crucial for normal biological function. In mammals, protein misfolding and aggregation leads to loss of function of the original protein while in many cases being associated with neurodegenerative diseases, eventually leading to death of the …
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Covalent post-translational modification of proteins in Escherichia coli: Genetic and biochemical studies of biotin and lipoic acid biosynthesis and function
I have identified four distinct classes of mutants involved in either lipoic acid biosynthesis or the covalent attachment of lipoic acid to proteins in E. coli. Two of these classes of mutants were isolated as Tn1000dKn insertion mutants and both classes map to min 14.5 on the E. coli chromosome. …
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An investigation of post-translational modification of human papillomavirus E4 and the role of modified E4 proteins during the virus life cycle
… the functional significance of these E1^E4 post-translational modifications during the HPV life cycle. This study has uncovered the novel finding that the E1^E4 protein of HPV type 18 (HPV18) exists as a phospho-protein within cells and is a substrate for multiple cellular kinases in vitro. …
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Can changes in SR protein acetylation affect alternative splicing?
… SR protein activity is regulated by the post-translational modification, phosphorylation. Another post-translational modification of SR proteins known as acetylation has recently been identified. The acetylation of lysine residues on proteins can result in changes in localization, …
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Unlocking thePadlock: Elucidation of Protein Arginine Deiminase 4 Function, Activity and Activation Dynamics
… (PADs 1, 2, 3, 4, and 6) that catalyze the post-translational modification of peptidyl-arginine to form peptidyl-citrulline in a variety of protein substrates. Over the past decade the importance of this post translational modification has become increasingly apparent as its putative roles …
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Investigating Antibiotic Biosynthesis Using Quadrupole-Fourier Transform Mass Spectrometry
… produced either nonribosomally or by extensive post-translational modification of a ribosomally-encoded precursor.
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