Infrared-monitored flash-photolysis of carboxymyoglobin

An infrared-monitored flash-photolysis apparatus capable of measuring absorbance changes to 10$\sp{-3}$OD over a time range from 10μs to 10s is described. Measurements made on this apparatus, combined with slower measurements made on a Fourier-transform infrared spectrometer, of the CO-rebinding kinetics of the A$\sb0$, A$\sb1$, and A$\sb3$ conformations of sperm-whale myoglobin (Mb) at atmospheric pressure and neutral pH are reported. The A$\sb0$, A$\sb1$, and A$\sb3$ rebinding kinetics are shown to be non-exponential and parameterized by activation-enthalpy distributions differing in prefactor k\sb0 (\log(k\sb0/s) = 10.8, 9.3, and 9.8 for the A$\sb0$, A$\sb1$, and A$\sb3$ conformations, respectively) and peak activation-enthalpy H\sb{p} (H\sb{p} = 10.4, 9.6, and 17.6 kJ/M, respectively).

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Physics

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Vittitow, Joseph Lawrence

• Physics, Molecular
• Biophysics, General

Statement dc:rights

• Copyright 1989 Vittitow, Joseph Lawrence

Language dc:language

eng

Identifier

AAI9011062
(UMI)AAI9011062

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/22089