{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/22089"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/22089","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Infrared-monitored flash-photolysis of carboxymyoglobin","abstract":"An infrared-monitored flash-photolysis apparatus capable of measuring absorbance changes to 10$\\sp{-3}$OD over a time range from 10$\\mu$s to 10s is described. Measurements made on this apparatus, combined with slower measurements made on a Fourier-transform infrared spectrometer, of the CO-rebinding kinetics of the A$\\sb0$, A$\\sb1$, and A$\\sb3$ conformations of sperm-whale myoglobin (Mb) at atmospheric pressure and neutral pH are reported. The A$\\sb0$, A$\\sb1$, and A$\\sb3$ rebinding kinetics are shown to be non-exponential and parameterized by activation-enthalpy distributions differing in prefactor $k\\sb0\\ (\\log(k\\sb0/s)$ = 10.8, 9.3, and 9.8 for the A$\\sb0$, A$\\sb1$, and A$\\sb3$ conformations, respectively) and peak activation-enthalpy $H\\sb{p}\\ (H\\sb{p} = 10.4$, 9.6, and 17.6 kJ/M, respectively).","abstract_html":"An infrared-monitored flash-photolysis apparatus capable of measuring absorbance changes to 10$\\sp{-3}$OD over a time range from 10<span class=\"etd-inline-math\">&mu;</span>s to 10s is described. Measurements made on this apparatus, combined with slower measurements made on a Fourier-transform infrared spectrometer, of the CO-rebinding kinetics of the A$\\sb0$, A$\\sb1$, and A$\\sb3$ conformations of sperm-whale myoglobin (Mb) at atmospheric pressure and neutral pH are reported. The A$\\sb0$, A$\\sb1$, and A$\\sb3$ rebinding kinetics are shown to be non-exponential and parameterized by activation-enthalpy distributions differing in prefactor <span class=\"etd-inline-math\">k\\sb0 (\\log(k\\sb0/s)</span> = 10.8, 9.3, and 9.8 for the A$\\sb0$, A$\\sb1$, and A$\\sb3$ conformations, respectively) and peak activation-enthalpy <span class=\"etd-inline-math\">H\\sb{p} (H\\sb{p} = 10.4</span>, 9.6, and 17.6 kJ/M, respectively).","abstract_has_math":true,"creators":["Vittitow, Joseph Lawrence"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Physics","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:28:35Z","date_published":"2011-05-07T13:28:35Z","updated_at":"2026-07-22T22:25:19Z","subjects":["Physics, Molecular","Biophysics, General"],"languages":["eng"],"rights":["Copyright 1989 Vittitow, Joseph Lawrence"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9011062","(UMI)AAI9011062"],"render_values":[{"text":"AAI9011062","href":null,"code":true},{"text":"(UMI)AAI9011062","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/22089","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Vittitow, Joseph Lawrence"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:28:35Z","10000-01-01","1989"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physics"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Physics, Molecular","Biophysics, General"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1989 Vittitow, Joseph Lawrence"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9011062","(UMI)AAI9011062","http://hdl.handle.net/2142/22089"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["An infrared-monitored flash-photolysis apparatus capable of measuring absorbance changes to 10$\\sp{-3}$OD over a time range from 10$\\mu$s to 10s is described. Measurements made on this apparatus, combined with slower measurements made on a Fourier-transform infrared spectrometer, of the CO-rebinding kinetics of the A$\\sb0$, A$\\sb1$, and A$\\sb3$ conformations of sperm-whale myoglobin (Mb) at atmospheric pressure and neutral pH are reported. The A$\\sb0$, A$\\sb1$, and A$\\sb3$ rebinding kinetics are shown to be non-exponential and parameterized by activation-enthalpy distributions differing in prefactor $k\\sb0\\ (\\log(k\\sb0/s)$ = 10.8, 9.3, and 9.8 for the A$\\sb0$, A$\\sb1$, and A$\\sb3$ conformations, respectively) and peak activation-enthalpy $H\\sb{p}\\ (H\\sb{p} = 10.4$, 9.6, and 17.6 kJ/M, respectively).","\"CO-rebinding kinetics of MbCO ensembles prepared along different paths in the pressure-temperature plane (\"\"freeze\"\" and \"\"squeeze-freeze-release\"\" ensembles) monitored at two frequencies within the A$\\sb1$ band are also reported. No differences between kinetics monitored at 1943.0cm$\\sp{-1}$ and 1947.5cm$\\sp{-1}$, and none between the kinetics of the freeze and squeeze-freeze-release ensembles, are resolved. The large uncertainty in the determination of the peak $H\\sb{p}$ of the activation-enthalpy distribution ($H\\sb{p}$ = 15 (+8,$-$7)kJ/M for the squeeze-freeze-release ensemble monitored at 1943.0cm$\\sp{-1}$) suggests limits of the capability of the apparatus, which are discussed in terms of time resolution.\"","Made available in DSpace on 2011-05-07T13:28:35Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9011062.pdf: 2085300 bytes, checksum: 03ce4ae399fb79ba187656caca234151 (MD5) Previous issue date: 1989","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:55:15Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:25:44-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Infrared-monitored flash-photolysis of carboxymyoglobin"]}]}],"canonical_facts":{"dc:creator":["Vittitow, Joseph Lawrence"],"dc:date":["2011-05-07T13:28:35Z","10000-01-01","1989"],"dc:description":["An infrared-monitored flash-photolysis apparatus capable of measuring absorbance changes to 10$\\sp{-3}$OD over a time range from 10$\\mu$s to 10s is described. Measurements made on this apparatus, combined with slower measurements made on a Fourier-transform infrared spectrometer, of the CO-rebinding kinetics of the A$\\sb0$, A$\\sb1$, and A$\\sb3$ conformations of sperm-whale myoglobin (Mb) at atmospheric pressure and neutral pH are reported. The A$\\sb0$, A$\\sb1$, and A$\\sb3$ rebinding kinetics are shown to be non-exponential and parameterized by activation-enthalpy distributions differing in prefactor $k\\sb0\\ (\\log(k\\sb0/s)$ = 10.8, 9.3, and 9.8 for the A$\\sb0$, A$\\sb1$, and A$\\sb3$ conformations, respectively) and peak activation-enthalpy $H\\sb{p}\\ (H\\sb{p} = 10.4$, 9.6, and 17.6 kJ/M, respectively).","\"CO-rebinding kinetics of MbCO ensembles prepared along different paths in the pressure-temperature plane (\"\"freeze\"\" and \"\"squeeze-freeze-release\"\" ensembles) monitored at two frequencies within the A$\\sb1$ band are also reported. No differences between kinetics monitored at 1943.0cm$\\sp{-1}$ and 1947.5cm$\\sp{-1}$, and none between the kinetics of the freeze and squeeze-freeze-release ensembles, are resolved. The large uncertainty in the determination of the peak $H\\sb{p}$ of the activation-enthalpy distribution ($H\\sb{p}$ = 15 (+8,$-$7)kJ/M for the squeeze-freeze-release ensemble monitored at 1943.0cm$\\sp{-1}$) suggests limits of the capability of the apparatus, which are discussed in terms of time resolution.\"","Made available in DSpace on 2011-05-07T13:28:35Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9011062.pdf: 2085300 bytes, checksum: 03ce4ae399fb79ba187656caca234151 (MD5) Previous issue date: 1989","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:55:15Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:25:44-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9011062","(UMI)AAI9011062","http://hdl.handle.net/2142/22089"],"dc:language":["eng"],"dc:rights":["Copyright 1989 Vittitow, Joseph Lawrence"],"dc:subject":["Physics, Molecular","Biophysics, General"],"dc:title":["Infrared-monitored flash-photolysis of carboxymyoglobin"],"dc:type":["text"],"thesis:degree_discipline":["Physics"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:19Z"}