Variation in proline-rich proteins and DNA methylation patterns in soybean seed color isolines

The I locus controls pattern expression of anthocyanin pigments in soybean seed coats. Using isolines of soybean cultivars varying only at the I locus, a seed coat cell wall protein that is affected by seed coat color was identified. A cDNA library was constructed using messenger RNA isolated from immature soybean seed coats. A 993 base pair clone corresponding to the protein was isolated and sequenced. The protein was identified as PRP1. It was abundant in immature seed coats of yellow seeded Richland but present at a much reduced level in the black seeded isoline T157. PRP1 was isolated from soybean seed coats using insoluble polyvinylpolypyrrolidone column chromatography. The expression of a similar seed coat protein, PRP2, was not affected by seed color genotype. Thus, a mutation in the anthocyanin pathway appears to affect a specific cell wall protein, PRP1.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Crop Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Lindstrom, Jon Tranell

Contributors dc:contributor

• Vodkin, Lila O.

• Agriculture, Agronomy
• Biology, Genetics

Statement dc:rights

• Copyright 1991 Lindstrom, Jon Tranell

Language dc:language

eng

Identifier

AAI9136664
(UMI)AAI9136664

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/21838