Molecular systematics and population genetics of Hypoxylon truncatum sensu Miller using RAPD

Molecular systematics and population genetics of Hypoxylon truncatum sensu Miller were investigated, using random amplified polymorphic DNA markers (RAPD). A simple, rapid method was developed to isolate DNA from fungal tissue. This method described involves treating ground mycelium with a lysis buffer containing SDS, followed by precipitation of contaminants using CsCl. Using DNA isolated by this method, the effects of several parameters on PCR amplification were investigated. These parameters included different concentrations of DNA, MgCl$\sb2,$ dNTP, gelatin, primer, and Taq polymerase, different temperature profiles, and presence of RNA. The result of this study suggest that concentrations of reaction components studied are highly inter-dependant in their effects, and that RNA can interfere with PCR amplification of target DNA sequences.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Crop Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Yoon, Cheol-Sik

Contributors dc:contributor

• Glawe, Dean A.

• Biology, Genetics
• Biology, Microbiology
• Agriculture, Plant Pathology

Statement dc:rights

• Copyright 1992 Yoon, Cheol-Sik

Language dc:language

eng

Identifier

AAI9305746
(UMI)AAI9305746

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/21786