{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/21786"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/21786","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Molecular systematics and population genetics of Hypoxylon truncatum sensu Miller using RAPD","abstract":"Molecular systematics and population genetics of Hypoxylon truncatum sensu Miller were investigated, using random amplified polymorphic DNA markers (RAPD). A simple, rapid method was developed to isolate DNA from fungal tissue. This method described involves treating ground mycelium with a lysis buffer containing SDS, followed by precipitation of contaminants using CsCl. Using DNA isolated by this method, the effects of several parameters on PCR amplification were investigated. These parameters included different concentrations of DNA, MgCl$\\sb2,$ dNTP, gelatin, primer, and Taq polymerase, different temperature profiles, and presence of RNA. The result of this study suggest that concentrations of reaction components studied are highly inter-dependant in their effects, and that RNA can interfere with PCR amplification of target DNA sequences.","abstract_html":"Molecular systematics and population genetics of Hypoxylon truncatum sensu Miller were investigated, using random amplified polymorphic DNA markers (RAPD). A simple, rapid method was developed to isolate DNA from fungal tissue. This method described involves treating ground mycelium with a lysis buffer containing SDS, followed by precipitation of contaminants using CsCl. Using DNA isolated by this method, the effects of several parameters on PCR amplification were investigated. These parameters included different concentrations of DNA, MgCl$\\sb2,$ dNTP, gelatin, primer, and Taq polymerase, different temperature profiles, and presence of RNA. The result of this study suggest that concentrations of reaction components studied are highly inter-dependant in their effects, and that RNA can interfere with PCR amplification of target DNA sequences.","abstract_has_math":true,"creators":["Yoon, Cheol-Sik"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Crop Sciences","degree_department":null,"school":null,"contributors":["Glawe, Dean A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:19:06Z","date_published":"2011-05-07T13:19:06Z","updated_at":"2026-07-22T22:25:18Z","subjects":["Biology, Genetics","Biology, Microbiology","Agriculture, Plant Pathology"],"languages":["eng"],"rights":["Copyright 1992 Yoon, Cheol-Sik"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9305746","(UMI)AAI9305746"],"render_values":[{"text":"AAI9305746","href":null,"code":true},{"text":"(UMI)AAI9305746","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/21786","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Glawe, Dean A."]},{"key":"dc:creator","label":"Author","values":["Yoon, Cheol-Sik"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:19:06Z","10000-01-01","1992"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Genetics","Biology, Microbiology","Agriculture, Plant Pathology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1992 Yoon, Cheol-Sik"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9305746","(UMI)AAI9305746","http://hdl.handle.net/2142/21786"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Molecular systematics and population genetics of Hypoxylon truncatum sensu Miller were investigated, using random amplified polymorphic DNA markers (RAPD). A simple, rapid method was developed to isolate DNA from fungal tissue. This method described involves treating ground mycelium with a lysis buffer containing SDS, followed by precipitation of contaminants using CsCl. Using DNA isolated by this method, the effects of several parameters on PCR amplification were investigated. These parameters included different concentrations of DNA, MgCl$\\sb2,$ dNTP, gelatin, primer, and Taq polymerase, different temperature profiles, and presence of RNA. The result of this study suggest that concentrations of reaction components studied are highly inter-dependant in their effects, and that RNA can interfere with PCR amplification of target DNA sequences.","A single population of H. truncatum from southern Illinois was studied. High degree of genetic variation was observed among single-ascospore isolates from a single stroma. Phenetically analyzed molecular data suggest that a clear association exists between RAPD markers and different stromatal types in the population studied. Another study investigated the genetic structure of six geographically distant populations. Phenetic analyses of a 99 character by 54 isolate matrix separated the 54 collections into two genetically distinct groups corresponding with different stromatal types. The results suggest that because the two stromatal forms of H. truncatum sensu Miller are genetically distinct, it would be useful to designate the different groups as different species. Phenogram branching patterns do not correspond with different populations studied, suggesting that gene flow likely occurs among these populations.","Made available in DSpace on 2011-05-07T13:19:06Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9305746.pdf: 4447965 bytes, checksum: 2bef0d35fc5bbbd4f41e3d1eb4cc1477 (MD5) Previous issue date: 1992","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:53:12Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:24:34-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Molecular systematics and population genetics of Hypoxylon truncatum sensu Miller using RAPD"]}]}],"canonical_facts":{"dc:contributor":["Glawe, Dean A."],"dc:creator":["Yoon, Cheol-Sik"],"dc:date":["2011-05-07T13:19:06Z","10000-01-01","1992"],"dc:description":["Molecular systematics and population genetics of Hypoxylon truncatum sensu Miller were investigated, using random amplified polymorphic DNA markers (RAPD). A simple, rapid method was developed to isolate DNA from fungal tissue. This method described involves treating ground mycelium with a lysis buffer containing SDS, followed by precipitation of contaminants using CsCl. Using DNA isolated by this method, the effects of several parameters on PCR amplification were investigated. These parameters included different concentrations of DNA, MgCl$\\sb2,$ dNTP, gelatin, primer, and Taq polymerase, different temperature profiles, and presence of RNA. The result of this study suggest that concentrations of reaction components studied are highly inter-dependant in their effects, and that RNA can interfere with PCR amplification of target DNA sequences.","A single population of H. truncatum from southern Illinois was studied. High degree of genetic variation was observed among single-ascospore isolates from a single stroma. Phenetically analyzed molecular data suggest that a clear association exists between RAPD markers and different stromatal types in the population studied. Another study investigated the genetic structure of six geographically distant populations. Phenetic analyses of a 99 character by 54 isolate matrix separated the 54 collections into two genetically distinct groups corresponding with different stromatal types. The results suggest that because the two stromatal forms of H. truncatum sensu Miller are genetically distinct, it would be useful to designate the different groups as different species. Phenogram branching patterns do not correspond with different populations studied, suggesting that gene flow likely occurs among these populations.","Made available in DSpace on 2011-05-07T13:19:06Z (GMT). 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