Development of genetic systems for Clostridia spp. by utilization of extrachromosomal DNA elements

An Escherichia coli-Clostridium perfringens shuttle vector (pAK201) was constructed by ligating a 3.6 Kb fragment of plasmid pBR322 with C. perfringens plasmid pHB101 (3.1 Kb). Plasmid pKA201 is 8 kb and codes for resistance to chloramphenicol in E. coli and C. perfringens. Plasmid pAK201 was transformed into E. coli and C. perfringens using electroporation. The transformation frequencies were 10$\sp6$ and 10$\sp4$ transformants/ug DNA in E. coli and C. perfringens.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Food Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Kim, Augustine Yonghwi

Contributors dc:contributor

• Witter, Lloyd D.

• Agriculture, Food Science and Technology
• Biology, Microbiology

Statement dc:rights

• Copyright 1992 Kim, Augustine Yonghwi

Language dc:language

eng

Identifier

AAI9215838
(UMI)AAI9215838

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/21678