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Showing 1 to 20 of 48 for “"C. perfringens"”.
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Holin-dependent secretion of a large clostridial cytotoxin by C. perfringens
… TcsL and TcsH in P. sordellii, and TpeL in C. perfringens. LCTs bind and inactivate small GTPases in host mammalian cells, shutting down cell-signaling and eventually leading to cell death. Intriguingly, LCTs all lack any conventional signal peptide, rendering them incapable of being …
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Development of a rapid molecular method for detection of C. perfringens and other pathogens in water sources
… to disinfection. Examples are Clostridium perfringens (a spore forming microorganism with a cell envelope that is less sensitive to chemical disinfection and the cause of diarrhoea via contaminated water and food) and Legionella pneumophila (a waterborne, pulmonary pathogen protected from …
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Ταχεία ανίχνευση της εντεροτοξίνης του C. perfringens τύπου Α με την ανοσοενζυμική μέθοδο ELISA, σε κόπρανα ασθενών με συμπτώματα τροφικής δηλητηρίασης
C. perfringens type A is one of the most common agents for the food poisonings in human, worldwide. Its enterotoxin produced during the sporulation is particularly considered to be responsible for the development of the food poisoning, symptoms. The detection of its enterotoxin in feces of patients …
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The Interactions of Clostridium Perfringens With Phagocytic Cells
Clostridium perfringens is the most common cause of gas gangrene (clostridial myonecrosis), a disease that begins when ischemic tissues become contaminated with C. perfringens. C. perfringens quickly multiplies in ischemic tissues and spreads to healthy areas, leading to high levels of morbidity …
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Investigation of the role of the toxins perfringolysin O (PFO) and sialidase in Clostridium perfringens gas gangrene infections
Clostridium perfringens is the causative agent of gas gangrene. A lethal infection in mice requires a large inoculum suggesting that the immune system is involved in inhibiting disease. Human monocytic cells and neutrophils killed C. perfringens in vitro when complement was present. Macrophages and …
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Development of genetic systems for Clostridia spp. by utilization of extrachromosomal DNA elements
An Escherichia coli-Clostridium perfringens shuttle vector (pAK201) was constructed by ligating a 3.6 Kb fragment of plasmid pBR322 with C. perfringens plasmid pHB101 (3.1 Kb). Plasmid pKA201 is 8 kb and codes for resistance to chloramphenicol in E. coli and C. perfringens. Plasmid pAK201 was …
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Development and application of a gene transfer system in Clostridium perfringens
… transformation of intact cells of C. perfringens 3624A-Rif$\sp{\rm r}$/Str$\sp{\rm r}$ with plasmids pAM$\beta$1 and pHR106 resulted in transformation efficiencies of 1.4 $\times$ 10$\sp2$ and 1.2 $\times$ 10$\sp3$ transformants/$\mu$g DNA, respectively. An examination of parameters …
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Characterization of the Components of Carbon Catabolite Repression in Clostridium perfringens
Clostridium perfringens is a versatile pathogen capable of causing a wide array of diseases, ranging from clostridial food poisoning to tissue infections such as gas gangrene. An important factor in virulence as well as in the distribution of C. perfringens is its ability to form an endospore. The …
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Validation of the Fung double tube to enumerate Clostridium perfringens from the intestinal contents of broiler chickens raised on different diets
Clostridium perfringens causes necrotic enteritis (NE), resulting in decreased feed efficiency and increased mortality, costing the poultry industry USD 2 billion a year worldwide. The objective of the first trial was to validate the Fung Double Tube (FDT) to detect and enumerate C. perfringens in …
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Factors Affecting the Heat Resistance of Clostridium perfringens Spores
The bacterium Clostridium perfringens is a gram-positive anaerobe responsible for many diseases in man and other animals, the most common of which is acute food poisoning (AFP). It is estimated that nearly 240,000 cases of AFP occur each year in the U.S. The C. perfringens spore plays an important …
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Enterococci and its enterocins as an alternative to antibiotics in poultry affected by necrotic enteritis
… to preserve human and animal health. Clostridium perfringens infections associated with necrotic enteritis (NE) are one of the major threats of the poultry sector, that requires a close surveillance. Antibiotic resistance in C. perfringens is emerging, but as this microorganism is not subject to …
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The Influence of Necrotic Enteritis, Environmental Factors, and Genetics on Intestinal Development Pathways and Disease Occurrence in Broiler Chickens
… chickens to a bacterial infection Clostridium perfringens. Intestinal structure, function, inflammatory response, and epithelial composition was examined during a mild subclinical infection. Analysis of E. maxima and C. perfringens as individual infections revealed how each pathogen contributes …
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The Role of CcpA in Regulating the Carbon-Starvation Response of Clostridium perfringens
Clostridium perfringens is a significant human pathogen, causing 250,000 cases of food poisoning in addition to several thousand potentially lethal cases of gas gangrene each year in the United States. Historically, work in this field has centered around toxin production, as C. perfringens can …
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Localization of Type IV Pilin Polymerization Proteins in Clostridium perfringens
Clostridium perfringens is a spore-forming anaerobic Gram-positive rod which has gliding motility through type IV Pili (TFP). Since the discovery of TFP in Gram-positive bacteria is relatively new, more studies are required to understand the mechanism and interaction of the proteins of this …
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Sporulation and enterotoxin regulation by sigma factors in Clostridium perfringens
Clostridium perfringens is a leading cause of food poisoning annually in the United States. Ingested C. perfringens vegetative cells respond to the acidic conditions of the stomach by initiating sporulation. The process of sporulation is essential in the formation of an enterotoxin (CPE) that is …
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Bacteriophage Endolysins: Anti-Infectives and Lysidiagnostics for the Post-Antibiotic ERA
… specifically <em>Clostridium perfringens</em> (<em>C. perfringens</em>). <em>Paenibacilli</em> are gram-positive, aerobic or facultatively anaerobic, spore-forming bacilli of the soil microbiome. While not traditionally considered human pathogens, they can cause …
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Investigating the Potential of Microbial Metabolites and mTOR Signaling on Chicken Necrotic Enteritis
<p>Necrotic enteritis (NE), caused by C. perfringens and coccidiosis, is responsible for substantial economic loss annually. In the first chapter, we investigated the effect of secondary bile acid on necrotic enteritis. Day-old broiler chicks were randomly assigned to 5 groups of diets supplemented …
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Identification of genes involved in gliding motility and proteomic analysis of spore inner membrane proteins in Clostridium perfringens
Clostridium perfringens is a Gram-positive anaerobic pathogen of humans and animals. While lacking flagella, C. perfringens cells can still migrate across surfaces using a type of gilding motility that involves the formation of filaments of bacteria lined up in an end to end conformation. To …
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Immunological Response to Clostridium perfringens in Two Genetically Divergent Lines of Chickens as Influenced by Major Histocompatibility Complex (MHC) Genotype
… LA and HA lines during exposure to Clostridium perfringens. In Experiment 1, chickens were orally gavaged with a low (10⁷ CFU/mL) or high (10⁹ CFU/mL) dose of C. perfringens. In Experiment 2, chickens were orally gavaged with live coccidia oocysts on experiment d 1, followed by 10⁷ CFU/mL C. …
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Molecular Analysis of Type IV Pilus Assembly in Clostridium perfringens
Clostridium perfringens is a Gram-positive anaerobe capable of causing disease in humans and many animals. C. perfringens is able to move across surfaces in a manner that is dependent on growth and type IV pili. Type IV pili are filaments that can be extended away from the cell by rapid …
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