Developmental and estrogen regulation of Xenopus laevis retinol-binding protein gene expression

The developmental regulation of the estrogen-regulated serum retinol-binding protein and estrogen receptor genes in Xenopus embryonic livers has been examined. Serum retinol binding protein (RBP) mRNA is detected exclusively in liver tissue in both adult Xenopus laevis and in tadpoles. RBP mRNA is absent in Xenopus oocytes and is present at extremely low levels in early embryos. The developmental activation of constitutive RBP gene expression parallels liver differentiation. In contrast, the capacity for estrogen induction of RBP gene transcription appears much later and is coordinate with the expression of the estrogen receptor (xER) gene. xER mRNA is expressed at extremely low levels up to stage 66, at which time there is a dramatic increase in constitutive expression and inducible expression becomes apparent for the first time. The constitutive level of hepatic xER mRNA at the completion of metamorphosis is approximately half the level in livers of uninduced adult male Xenopus, but is still sufficient for the acquisition of estrogen inducibility.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Gould, Lisa Jeanne

Contributors dc:contributor

• Shapiro, David J.

• Biology, Molecular

Statement dc:rights

• Copyright 1989 Gould, Lisa Jeanne

Language dc:language

eng

Identifier

AAI8916252
(UMI)AAI8916252

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/21628