The molecular mechanisms of transcription termination and pausing in the Escherichia coli threonine operon regulatory region

To study the mechanism of transcription termination at a rho-independent terminator, homoduplex and heteroduplex templates that contained mutations in the G + C-rich region of dyad symmetry in the Escherichia coli thr operon attenuator were constructed. Assays of these templates in an in vitro transcription system showed that the DNA sequence of the template strand determines the transcription termination efficiency. There was no indication that the potential complementary base pairs between the non-transcribed DNA strand and the nascent RNA in the G + C-rich region are involved in transcription termination. The results argue against models where DNA-DNA pairing or RNA pairing with the non-transcribed DNA strand in a bridged intermediate are important for transcription termination.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Yang, Ming-Te

Contributors dc:contributor

• Gardner, Jeffrey F.

• Biology, Molecular
• Biology, Microbiology

Statement dc:rights

• Copyright 1990 Yang, Ming-Te

Language dc:language

eng

Identifier

AAI9114472
(UMI)AAI9114472

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/21426