Studies of a DNA modification methyltransferase from Rhodobacter sphaeroides

The DNA methyltransferases represent an interesting class of enzymes for the study of protein-DNA interactions due to their high specificity, structural simplicity, and biological importance. RsrI methyltransferase (M$\cdot$RsrI) from Rhodobacter sphaeroides recognizes duplex d(GAATTC) and deposits methyl groups at the $N\sp6$ position of central adenine. M$\cdot$RsrI was purified to homogeneity from R. sphaeroides, and its gene cloned and sequenced. The purification used four chromatography columns, and yielded up to 100 μg of enzyme. M$\cdot$RsrI was overexpressed in E. coli and the yield of the purification improved by about 100-fold with respect to that from R. sphaeroides.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

1991

Author dc:creator

• Kaszubska, Wiweka

Contributors dc:contributor

• Gumport, Richard I.

• Chemistry, Biochemistry

Statement dc:rights

• Copyright 1991 Kaszubska, Wiweka

Language dc:language

eng

Identifier

AAI9124438
(UMI)AAI9124438

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/21056