Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"DNA modification"”.
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Studies of a DNA modification methyltransferase from Rhodobacter sphaeroides
The DNA methyltransferases represent an interesting class of enzymes for the study of protein-DNA interactions due to their high specificity, structural simplicity, and biological importance. RsrI methyltransferase (M$\cdot$RsrI) from Rhodobacter sphaeroides recognizes duplex d(GAATTC) and deposits …
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The HLA Complex Group 9 Gene Modification Study in Major Psychosis
… psychosis, conducted by our group, identified DNA modification differences at HLA complex group 9 (non-protein coding) gene (HCG9) in the post-mortem brain samples of individuals affected with schizophrenia (SCZ) and bipolar disorder (BPD). In this thesis we present results from bisulfite …
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Methods for the development of a DNA based nanoelectronics
The exceptional self-assembly properties of DNA as well as its ability to interact with different kinds of chemical compounds and biological structures make this biomolecule to an interesting object for the fabrication of artificial nanostructures. In this work several methods for a DNA-based …
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Effects of m6A DNA methylation by bacterial methyltransferase in colorectal cancer
<p>Effects of m6A DNA methylation by bacterial methyltransferase in colorectal cancer Fabian Alejandro Mendoza Galvan Advisory Professor: Angela H. Ting, Ph.D. Fusobacterium nucleatum animalis (Fna) is found in the human oral cavity and gut. A distinct clade of Fna is primarily enriched in the …
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Effects of Long-term Strenuous Exercise on Oxidative DNA Damage and Proteinuria in Humans
… of long-term strenuous exercise on oxidative DNA damage and proteinuria in trained individuals. In the first project, four successive triathlons accompanied by long-term training in a triathlete was chosen as the experimental design and procedure. In the second project, repeated 5-h bouts of …
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Effects of Long-term Strenuous Exercise on Oxidative DNA Damage and Proteinuria in Humans
… of long-term strenuous exercise on oxidative DNA damage and proteinuria in trained individuals. In the first project, four successive triathlons accompanied by long-term training in a triathlete was chosen as the experimental design and procedure. In the second project, repeated 5-h bouts of …
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Nucleic acid modifications in bacterial pathogens - impact on pathogenesis, diagnosis, and therapy
Nucleic acids are subject to extensive chemical modification by all organisms. These modifications display incredible structural diversity, and some are essential for survival. Intriguingly, several of these modifications are unique to bacteria, including many human pathogens. Given the enormous …
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The DNA Binding Protein Lsr2 from Mycobacterium tuberculosis
Lsr2 is a small, basic DNA binding protein that is highly conserved in mycobacteria and related actinomycetes. Lsr2 is essential for growth in Mycobacterium tuberculosis and previous studies have shown that Lsr2 is involved in down-regulating a wide range of genes involved in cell wall synthesis …
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Deoxyribonucleic acid restriction/modification systems and gene transfer strategies in Ruminococcus albus 8 and Ruminococcus flavefaciens FD-1
One goal critical to the use of recombinant DNA technologies with rumen bacteria is the establishment of a stable DNA transfer system. Research to date has shown that Ruminococcus can be classified as a genus resistant to transformation, and that electroporation may offer the only means to …
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Exploring functional significance of asymmetric DNA methylation in early mammalian development
DNA methylation is an epigenetic modification important for the regulation of transcriptional activity in processes like genomic imprinting, retrotransposon silencing and centromeric stabilisation. It is also crucial for correct embryonic development and the differentiation of embryonic stem cells …
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Statistical and computational approaches for the analysis of high-throughput epigenomic data /
Epigenetic mechanisms, such as DNA modifications, are of great importance in all living things. Despite the importance of DNA modifications, many of the difficulties involved in establishing and characterizing epigenetic profiles discourage researchers from following this path of research. TOP-seq …
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Covalent DNA Modifications in Phage and Bacterial Dynamics
… enzymes and CRISPR systems, and the covalent DNA modifications that neutralize them. Multiple targeted nucleases equip bacteria with an innate immune response against phage, and CRISPR systems provide an adaptive immune response. I report three main studies. 1) To study the human gut …
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The Life of Prion: an investigation into the physiological role of a prion-like protein in the nematode Caenorhabditis elegans
… for biological advantage without the need for DNA modification. The properties observed in many of these proteins, including those of multivalency and low complexity, predispose them towards phase separation, whereby localised concentration of biomolecules compartmentalise cellular activity. …
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Anisotropic surface functionalization and applications of upconversion nanoparticles
… spherical in shape and that isotropic surface modification strategies such as ligand oxidation, amphiphilic ligand interaction (insert), ligand exchange, and silica coating techniques are sufficient for their surface modification. In this thesis, the author explores the surface coverage at the …
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Chemistry and Biology of DNA-protein cross-links
DNA-protein cross-links (DPCs) are ubiquitous DNA lesions formed when proteins become covalently trapped on DNA strands upon exposure to various endogenous, environmental and chemotherapeutic agents. Because of their considerable size, DPCs interfere with the progression of replication and …
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Metal-cyclam based Metal-Organic Frameworks for CO₂ Chemical Transformations
… absorption in visible spectrum, oxygen-mediated DNA and BSA photocleavage and significant photocytotoxicity under blue light irradiation along with negligible activity in the dark. The compounds show approximately five-fold higher cytotoxicity compared the traditional chemotherapeutic drug, …
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CHEMICAL BIOLOGY STRATEGIES FOR THE FORMATION AND CLEAVAGE OF CHEMICAL BONDS
… preparation of site-specific and plasma stable DNA-protein conjugates which are suitable for cellular applications. Using the method developed, various types of ONs can be easily conjugated to a range of proteins via solvent accessible cysteine residues, including therapeutic full-length IgG …
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Probing the Redox and Photophysical Properties of Ru(II)-Pt(II) Supramolecular Complexes as Efficient Photodynamic Therapy Agents
… bimetallic complexes in light activated DNA modification and cytotoxicity were evaluated. The supramolecular design entails covalently coupling an efficient Ru(II) chromophore for photodynamic therapy (PDT) activity through a polyazine bridging ligand (dpp or dpq) to a cis-PtCl₂ bioactive …