The autoregulatory and tissue-specific activation of gene expression by estrogen receptor

A single, transient dose of estradiol-17β is sufficient to elicit the permanent induction of hepatic estrogen receptor mRNA, which is induced 18-fold, from 0.13 molecules/cell to 2.4 molecules/cell, and then remains fully induced for at least 125 days. In primary liver cultures, extremely low concentrations of estradiol-17β, which are below the K$\sb{\rm d}$ of the Xenopus laevis estrogen receptor, maintain persistent induction of estrogen receptor mRNA, but not of estrogen-inducible vitellogenin mRNA. This data, and the ability of the antiestrogen, hydroxytamoxifen, to reverse persistent induction of estrogen receptor mRNA, support a model in which transient doses of estradiol-17β induce the estrogen receptor and thereby establish an autoregulatory loop. The low levels of estradiol-17β normally circulating in male Xenopus laevis and the elevated level of receptor provide sufficient hormone-receptor complex to permanently maintain the induced level of expression of the estrogen receptor gene. The irreversible induction of the estrogen receptor may be the regulatory switch which results in the persistent expression of a recently identified class of proteins which exhibit long-term responses to estrogen.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Barton, Michelle Craig

Contributors dc:contributor

• Shapiro, David J.

• Biology, Molecular

Statement dc:rights

• Copyright 1989 Barton, Michelle Craig

Language dc:language

eng

Identifier

AAI8916213
(UMI)AAI8916213

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/20470