Sex-specific expression of CYP2C2 in transgenic mice and phenobarbital regulation of CYP2B by in situ injection

To study the transcriptional regulation of CYP2C2, transgenic mice were generated using a DNA fragment containing 3500bp of CYP2C2 5$\sp\prime$-flanking region fused to a luciferase reporter gene. In one of three transgenic lines, this transgene was expressed in the liver and at a lower level in the kidney, not in the lung, spleen, testis, intestine, or heart, similar to the tissue-specific pattern of this gene in rabbits. Unlike expression in rabbits, the transgene was expressed in a male-specific pattern in liver and kidney, and was undetectable in the female. Expression in the male mice was repressed after castration, while testosterone could partially reverse the repression. The expression in females was stimulated after testosterone treatment and periodic injection of rat growth hormone (GH). The expression of the transgene was detected in 5-6 week-old mice and reached a high level at 8 weeks. Unlike in rabbit, this transgene was also detected in the brain, and the brain expression was not sex-specific. These results suggest that this 3500bp of CYP2C2 5$\sp\prime$-flanking region contains the basal transcriptional regulation elements for tissue-specific and developmental expression. This transgene may include the cis-regulatory element for sexual dimorphic expression or a nearby enhancer in the mouse genome may be responsible (position effect).

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Li, Hong

Contributors dc:contributor

• Kemper, Byron W.

• Biology, Molecular

Statement dc:rights

• Copyright 1996 Li, Hong

Language dc:language

eng

Identifier

9780591199161
AAI9712351
(UMI)AAI9712351

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/20339