Characterization of SEC14: A gene essential for secretion in Saccharomyces cerevisiae

SEC14, a Saccharomyces cerevisiae gene whose product is required for export of yeast secretory proteins from the Golgi complex, was cloned and characterized. SEC14 was shown to be an essential gene for vegetative growth. Nucleotide sequencing revealed SEC14 to be an intron-containing gene, which encodes a 35 kD protein. Sec14p was demonstrated to be nonglycosylated, and to behave primarily as a cytosolic protein. Structurally related proteins were detected in yeasts Schizosaccharomyces pombe and Kluyveromyces lactis. The homologous Sec14p from Kluyveromyces lactis was shown to functionally complement sec14$\sp{\rm ts}$ defects in S. cerevisiae, suggesting a degree of conservation of Sec14p structure and function.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Malehorn, David Edward

• Biology, Molecular

Statement dc:rights

• Copyright 1990 Malehorn, David Edward

Language dc:language

eng

Identifier

AAI9026261
(UMI)AAI9026261

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/20146