Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 23 for “"Golgi complex"”.
-
Studies on the structural organization of Golgi complex
Golgi complex is a multi-compartmental organelle involved in posttranslational modification and sorting of secretory proteins. We have characterized the distribution in Vero cells of three Golgi cisternal membrane proteins, Sialyltransferase (SialylT, tans- Golgi/TGN), galactosyltransferase(GalT, …
-
The Golgi Complex of the Hepatocyte: Isolation and Partial Enzomological Characterization
<p>The elements of the Golgi complex of the hepatocyte are characterized by their content of 30-80m osmiophilic particles, which have been identified by others as very low density lipoprotein. The vast majority of such particles in the cell are in the elements of the Golgi complex. Moreover, the …
-
Characterization of a Novel GPI-anchored Protein, a Component of Sphingomyelin-enriched Microdomains at the Golgi Complex
The Golgi complex is the central station along the secretory pathway to sort proteins and lipids to their final destinations. It exists of a series of flattened cisternal membranes that are aligned in parallel to form polarized stacks. This structure is actively maintained amid a large flow of …
-
The Role of ER-Golgi Membrane Contact Sites and of FAPP1 in Phosphoinositide Homeostasis at the Golgi Complex
… and cell migration. PI4P levels at the Golgi in control conditions are the result of the balanced activity of Golgi-localized PI4-kinases (PI4KIIIβ and to a lesser extent PI4KIIα) and a single, highly conserved 4-phosphatase known as SAC1, localized in the ER. To coordinate such a …
-
Structural and Functional Characterization of GAPR-1, a Mammalian Plant Pathogenesis-related Protein in Lipid-enriched Microdomains of the Golgi Complex
… of lipid-enriched microdomains at the Golgi (GICs) (Gkantiragas, I. et al. 2001), a protein with an apparent molecular mass of 17 kDa was identified. Cloning and preliminary biochemical characterization identified a novel protein, GAPR-1, belonging to the superfamily of PR proteins. …
-
ER to Golgi transport during apoptosis
The Golgi complex, a single -copy organelle, is composed of a ribbon of flattened stacks of cisternae during interphase. However, during apoptosis the Golgi is shown to fragment and disperse throughout the cytoplasm. Immunoelectron microscopy identified these fragments as vesicular-tubular …
-
Cellular Trafficking and Processing Pathways of the Alzheimer β/A4 Amyloid Precursor Protein
… rat brain sections, APP was concentrated in the Golgi complex and in proximal axon segments. By immunoelectron microscopy of rat brain tissue fragments, APP was found associated with Golgi elements and with medium-sized, invaginated vesicles in both axons and dendrites. Prominent localization of …
-
Characterization of SEC14: A gene essential for secretion in Saccharomyces cerevisiae
… for export of yeast secretory proteins from the Golgi complex, was cloned and characterized. SEC14 was shown to be an essential gene for vegetative growth. Nucleotide sequencing revealed SEC14 to be an intron-containing gene, which encodes a 35 kD protein. Sec14p was demonstrated to be …
-
Characterization of Novel Neuronal Antigens in the Central Nervous System of Manduca Sexta
The predicted amino acid sequence of Ms-golgin80 is similar to several members of a growing family of coiled-coil, Golgi-associated proteins, including GM-130 and golgin-95. The Drosophila and Caenorhabditis elegans homologs of Ms-golgin80 were also identified through homology sequence search. …
-
Biochemical and Cellular Characterization of NMN Adenylyltransferase 2: A Brain Specific Isoform of an Essential NAD Synthesizing Enzyme
… expressed. Furthermore, Nmnat2 localizes to the Golgi complex in HeLa cells. Taken together, these observations suggest that Nmnat2 may have a specialized role in NAD metabolism in the brain. The goal of the work presented here is essentially to describe the molecular interactions of Nmnat2 and …
-
CELLULAR AND MOLECULAR PATHWAYS CONTROLLING SARS-COV2 ASSEMBLY AND EGRESS
… This feature adds an additional layer of complexity to the study of the coronavirus life cycle as, once assembled, virions have to traffic through host intracellular membranes to reach the plasma membrane to be released. The assembly of SARS-CoV-2 virions occurs at the ER-Golgi …
-
The role of recombinant human laminin-111 in adhesion-signaling and glycosylation in laminin-⍺2 deficient muscle
… altered localization of the laminin adhesion complexes to the sarcolemma and led us to explore the glycan composition of the dyW and other dystrophic mouse models utilizing different types of lectins. These results suggest that laminin-deficient muscle experiences dysfunctional Golgi complex …
-
Localisation of Protein Kinase C in Apoptosis and Neurite Outgrowth
… depends on the localisation of PKCqRD to the Golgi complex, which is mediated by the C1b domain of the protein. Mutation of a single amino acid residue, Met-267 in PKCqC1b, blocks both the Golgi localisation and the apoptotic effect of the PKCqRD. Previous studies have shown that PKCe induces …
-
The role of phospholipase D1 in trafficking and processing of amyloid precursor protein
… a primary station for amyloidogenesis, to the Golgi complex, while PLD1 overexpression produces the converse phenotype. Pld1 null mice harboring familial AD-linked (FAD) APP mutations exhibited decreased brain amyloid and an accumulation of APP COOH-terminal fragments. This finding was …
-
The SEC9 gene is essential for exocytosis in yeast and encodes a dispensable amino terminal domain
… for transport of secretory vesicles from the Golgi complex to the plasma membrane. The SEC9 gene was cloned from a yeast genomic library by complementation of the temperature sensitive growth defect of sec9-4 cells. The SEC9 gene is essential for viability in yeast and maps near the centromere …
-
Cell Biology of the ICA69 protein family in Neurosecretory cells
… directed against proteins associated with Golgi and post-Golgi secretory vesicles, including insulin secretory granules and synaptic-like microvesicles. Thus, the study of beta-cell autoantigens with yet unknown function may provide novel insight into the secretory machinery of beta-cells …
-
Mechanisms for Targeting of Proteins to Secretory Lysosomes of Haematopoietic Cells
… the secretory pathway, possibly operating in the Golgi complex and at the plasma membrane. In addition, the results indicated the suggested post-ER protein quality controls to eliminate proteins from the cell by constitutive secretion, instead of targeting failing proteins for intracellular …
-
Steps to Reconstitute in vitro a Complete Round of COPI vesicle Budding, Uncoating and Fusion
… anterograde and retrograde transport within the Golgi complex (Orci et al., 1997) as well as recycling of proteins from the Golgi to the ER (Cosson and Letourneur, 1994; Letourneur et al., 1994). An in vitro based assay using soluble coatomer and ARF1 together with synthetic liposomes containing …
-
DISSECTING THE ROLE OF GOLGI TRAFFIC IN BRAIN DEVELOPMENT ACROSS HEALTH AND DISEASE: A LESSON FROM COG5-CDG STUDIES
… and intracellular architecture. Interestingly, Golgi apparatus (GA) is differentially positioned in APs and BPs. GA is the main hub for glycosylation and defects in GA and associated glycosylation are observed in a group of rare multi system diseases collectively referred to as Congenital …
-
The Nuclear Pore Complex: Novel Glycoprotein Constituents
<p>Nuclear pore complexes are large, uniform organelles with a mass estimated to be on the order of 10<sup>8</sup> daltons. They are ubiquitous components of the nuclear envelope, providing a channel through which macromolecular transport between the nucleus and cytoplasm occurs. Thus far, almost …
Page 1 of 2