Regeneration and germination of cucumber seeds in vitro and a study of delayed flowering gene (df)

Old and fresh seeds of various cucumber (Cucumis sativus L.) lines were studied for germination and cotyledon regeneration. Depending on cultivars, seeds stored at 68$\sp\circ$F and 38% relative humidity remained 40 to 90% viable for 13 years. Seeds older than that were nonviable. It was also found that removing 1/4 cotyledon from fresh cucumber seed increased root number, length and hypocotyl growth. To develop an efficient cotyledon regeneration system, shoot regeneration from 1/6, 1/4, 1/2, 3/4 cotyledon segments was compared with shoot regeneration frequency of full cotyledon. It was found that shoot regeneration efficiency of full and 3/4 segments was statistically low compared with the rest of the treatments. No statistical differences were found in regenerability of 1/6, 1/4, and 1/2 segments of cucumber cotyledon. It was also found that position of segment relative to embryonic axis has no effect on shoot regeneration. It could be that endogenous growth regulators are evenly distributed in cucumber cotyledon. MS medium with kinetin (3 mg/L) enhanced the later growth of regenerated shoots.

Name thesis:degree_name

Ph.D. (doctoral)

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Crop Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Ali, Nawab

Contributors dc:contributor

• Skirvin, Robert M.
• Splittstoesser, Walter E.

• Biology, Genetics
• Agriculture, Plant Culture

Statement dc:rights

• Copyright 1990 Nawab Ali

Language dc:language

eng

Identifier

AAI9026119
(UMI)AAI9026119

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/20076