{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/20076"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/20076","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Regeneration and germination of cucumber seeds in vitro and a study of delayed flowering gene (df)","abstract":"Old and fresh seeds of various cucumber (Cucumis sativus L.) lines were studied for germination and cotyledon regeneration. Depending on cultivars, seeds stored at 68$\\sp\\circ$F and 38% relative humidity remained 40 to 90% viable for 13 years. Seeds older than that were nonviable. It was also found that removing 1/4 cotyledon from fresh cucumber seed increased root number, length and hypocotyl growth. To develop an efficient cotyledon regeneration system, shoot regeneration from 1/6, 1/4, 1/2, 3/4 cotyledon segments was compared with shoot regeneration frequency of full cotyledon. It was found that shoot regeneration efficiency of full and 3/4 segments was statistically low compared with the rest of the treatments. No statistical differences were found in regenerability of 1/6, 1/4, and 1/2 segments of cucumber cotyledon. It was also found that position of segment relative to embryonic axis has no effect on shoot regeneration. It could be that endogenous growth regulators are evenly distributed in cucumber cotyledon. MS medium with kinetin (3 mg/L) enhanced the later growth of regenerated shoots.","abstract_html":"Old and fresh seeds of various cucumber (Cucumis sativus L.) lines were studied for germination and cotyledon regeneration. Depending on cultivars, seeds stored at 68$\\sp\\circ$F and 38% relative humidity remained 40 to 90% viable for 13 years. Seeds older than that were nonviable. It was also found that removing 1/4 cotyledon from fresh cucumber seed increased root number, length and hypocotyl growth. To develop an efficient cotyledon regeneration system, shoot regeneration from 1/6, 1/4, 1/2, 3/4 cotyledon segments was compared with shoot regeneration frequency of full cotyledon. It was found that shoot regeneration efficiency of full and 3/4 segments was statistically low compared with the rest of the treatments. No statistical differences were found in regenerability of 1/6, 1/4, and 1/2 segments of cucumber cotyledon. It was also found that position of segment relative to embryonic axis has no effect on shoot regeneration. It could be that endogenous growth regulators are evenly distributed in cucumber cotyledon. MS medium with kinetin (3 mg/L) enhanced the later growth of regenerated shoots.","abstract_has_math":true,"creators":["Ali, Nawab"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D. (doctoral)","degree_level":"Dissertation","degree_discipline":"Crop Sciences","degree_department":null,"school":null,"contributors":["Skirvin, Robert M.","Splittstoesser, Walter E."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:28:03Z","date_published":"2011-05-07T12:28:03Z","updated_at":"2026-07-22T22:25:15Z","subjects":["Biology, Genetics","Agriculture, Plant Culture"],"languages":["eng"],"rights":["Copyright 1990 Nawab Ali"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9026119","(UMI)AAI9026119"],"render_values":[{"text":"AAI9026119","href":null,"code":true},{"text":"(UMI)AAI9026119","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/20076","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Skirvin, Robert M.","Splittstoesser, Walter E."]},{"key":"dc:creator","label":"Author","values":["Ali, Nawab"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:28:03Z","10000-01-01","1990"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D. (doctoral)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Genetics","Agriculture, Plant Culture"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1990 Nawab Ali"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9026119","(UMI)AAI9026119","http://hdl.handle.net/2142/20076"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Old and fresh seeds of various cucumber (Cucumis sativus L.) lines were studied for germination and cotyledon regeneration. Depending on cultivars, seeds stored at 68$\\sp\\circ$F and 38% relative humidity remained 40 to 90% viable for 13 years. Seeds older than that were nonviable. It was also found that removing 1/4 cotyledon from fresh cucumber seed increased root number, length and hypocotyl growth. To develop an efficient cotyledon regeneration system, shoot regeneration from 1/6, 1/4, 1/2, 3/4 cotyledon segments was compared with shoot regeneration frequency of full cotyledon. It was found that shoot regeneration efficiency of full and 3/4 segments was statistically low compared with the rest of the treatments. No statistical differences were found in regenerability of 1/6, 1/4, and 1/2 segments of cucumber cotyledon. It was also found that position of segment relative to embryonic axis has no effect on shoot regeneration. It could be that endogenous growth regulators are evenly distributed in cucumber cotyledon. MS medium with kinetin (3 mg/L) enhanced the later growth of regenerated shoots.","Gene df, found in 'Baroda' cucumber, has been associated with delayed flowering and delayed germination. The effect of this gene was studied in two cucumber lines ('Baroda' and 'Marketer'). It was found that the gene df alone is not responsible for inducing dormancy. The reported dormancy could be due to the interaction of df with modifiers or some other genetic or cytoplasmic factors. It was found that the mechanism of dormancy lies in the seed testa. This seed coat layer either restricts water uptake or, most likely, is impermeable to gas exchange. Dormancy, however, can be broken by puncturing this thin layer.","Made available in DSpace on 2011-05-07T12:28:03Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9026119.pdf: 3845459 bytes, checksum: 582cb34cb43d265ab2db580f599666a9 (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:41:24Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:17:54-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Regeneration and germination of cucumber seeds in vitro and a study of delayed flowering gene (df)"]}]}],"canonical_facts":{"dc:contributor":["Skirvin, Robert M.","Splittstoesser, Walter E."],"dc:creator":["Ali, Nawab"],"dc:date":["2011-05-07T12:28:03Z","10000-01-01","1990"],"dc:description":["Old and fresh seeds of various cucumber (Cucumis sativus L.) lines were studied for germination and cotyledon regeneration. Depending on cultivars, seeds stored at 68$\\sp\\circ$F and 38% relative humidity remained 40 to 90% viable for 13 years. Seeds older than that were nonviable. It was also found that removing 1/4 cotyledon from fresh cucumber seed increased root number, length and hypocotyl growth. To develop an efficient cotyledon regeneration system, shoot regeneration from 1/6, 1/4, 1/2, 3/4 cotyledon segments was compared with shoot regeneration frequency of full cotyledon. It was found that shoot regeneration efficiency of full and 3/4 segments was statistically low compared with the rest of the treatments. No statistical differences were found in regenerability of 1/6, 1/4, and 1/2 segments of cucumber cotyledon. It was also found that position of segment relative to embryonic axis has no effect on shoot regeneration. It could be that endogenous growth regulators are evenly distributed in cucumber cotyledon. MS medium with kinetin (3 mg/L) enhanced the later growth of regenerated shoots.","Gene df, found in 'Baroda' cucumber, has been associated with delayed flowering and delayed germination. The effect of this gene was studied in two cucumber lines ('Baroda' and 'Marketer'). It was found that the gene df alone is not responsible for inducing dormancy. The reported dormancy could be due to the interaction of df with modifiers or some other genetic or cytoplasmic factors. It was found that the mechanism of dormancy lies in the seed testa. This seed coat layer either restricts water uptake or, most likely, is impermeable to gas exchange. Dormancy, however, can be broken by puncturing this thin layer.","Made available in DSpace on 2011-05-07T12:28:03Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9026119.pdf: 3845459 bytes, checksum: 582cb34cb43d265ab2db580f599666a9 (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:41:24Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:17:54-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9026119","(UMI)AAI9026119","http://hdl.handle.net/2142/20076"],"dc:language":["eng"],"dc:rights":["Copyright 1990 Nawab Ali"],"dc:subject":["Biology, Genetics","Agriculture, Plant Culture"],"dc:title":["Regeneration and germination of cucumber seeds in vitro and a study of delayed flowering gene (df)"],"dc:type":["text"],"thesis:degree_discipline":["Crop Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D. (doctoral)"],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:15Z"}