Structure and expression of nuclear genes encoding light-harvesting chlorophyll a/b-binding polypeptides of photosystem II in apple and soybean

An apple genomic library was constructed with lambda Charon 35 vector. The library was screened using a pea LHCP-II cDNA and an apple LHCP-II gene (AB10) was identified, subcloned and characterized. AB10 contains an open reading frame of 807 nucleotides coding for a precursor of 269 amino acids divided by a transit peptide of 40 amino acids and a mature polypeptide of 229 amino acids. This gene also contains a TTGTTT-like polyadenylation signal, and a 5$\sp\prime$-promoter region including TATA-like and CACAT-like boxes. Comparisons between AB10 and other LHCP-II genes from various plant species indicate that all the LHCP-II genes have similarities, on the average, of about 70% at the nucleotide level and of about 80% at the amino acid level. The transit peptide sequence of AB10 differs from those of other LHCP-II genes of annual plants, suggesting more codon substitutions in AB10 transit peptide than silent base substitutions in those of the other LHCP-II genes.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Crop Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Chen, Houqi

Contributors dc:contributor

• Korban, Schuyler S.

• Biology, Molecular
• Biology, Genetics
• Biology, Plant Physiology

Statement dc:rights

• Copyright 1990 Chen, Houqi

Language dc:language

eng

Identifier

AAI9021662
(UMI)AAI9021662

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/19991