Chemiluminescence detection of NADH and enzyme substrates with immobilized tris(2,2'-bipyridyl)ruthenium (II) and dehydrogenase enzymes

The objective of this work is to couple the sensitivity of tris(2,2$\sp\prime$-bipyridyl)ruthenium(II) (Ru(bpy)$\sb3\sp{2+}$) electrogenerated chemiluminescence (ECL) to the selectivity of dehydrogenase enzymes. Within this objective, methods of reagent immobilization for Ru(bpy)$\sb3\sp{2+}$ and dehydrogenase enzyme have been studied and applied to bioanalytical determinations. Dehydrogenase enzymes employ NAD$\sp+$ as their cofactor and produce NADH in an amount equivalent to reacted analyte. Detection of NADH via the ECL reaction with Ru(bpy)$\sb3\sp{3+}$ is then used to determine analyte concentration.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Martin, Alice Fay

Contributors dc:contributor

• Nieman, Timothy A.

• Chemistry, Analytical
• Chemistry, Biochemistry

Statement dc:rights

• Copyright 1995 Martin, Alice Fay

Language dc:language

eng

Identifier

AAI9543667
(UMI)AAI9543667

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/19869