The identification and characterization of two murine genes regulated by the level of dietary fat

We have investigated the influence of the level of dietary fat on hepatic stearoyl CoA desaturase 1 (Scd1) and Low Fat Mammary 1 (Lfm1) expression and describe the structural organization of the Lfm1 gene. Scd1 and Lfm1 were isolated from a cDNA library constructed from mammary gland tissue of BALB/c mice fed a low fat diet (Elliott et al. 1993. Biochim. Biophys. Res. Comm. 190: 167-174). Northern hybridization analyses of hepatic total RNA from BALB/c mice fed diets containing 0, 5, 10, 15 or 20% corn oil showed a significant dose-dependent decrease in Scd1 mRNA abundance. Increasing the concentrations of dietary fat from 0-5% to 10, 15 or 20% significantly ($P<$ 0.05) decreased Scd1 mRNA by 50%, 75%, and 82%, respectively. The time course of Scd1 mRNA induction and the effects of fasting and refeeding on its abundance were examined in BALB/c mice fed diets containing 3, 10 or 20% corn oil. Hepatic Scd1 mRNA expression decreased after 12 h of fasting but increased 4-fold 6 h after refeeding in the 3% fat group compared to the 20% fat group. The abundance of Scd1 mRNA was also determined in response to type and amount of dietary fat in two inbred strains of mice with different susceptibilities to disease. Scd1 mRNA expression significantly ($P<$ 0.05) decreased in BALB/c mice fed diets containing 20% corn or coconut oil compared to those fed 4% of these fats in otherwise identical diets. C57BL/6 mice, which are more susceptible to atherosclerosis and type II diabetes compared to BALB/c mice, showed a significant ($P<$ 0.05) decrease in Scd1 mRNA abundance when fed diets containing 20% corn oil compared to 4% corn oil. In contrast, the level of Scd1 mRNA abundance remained unchanged by 4 and 20% coconut oil.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Nutritional Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Swartz, Deborah Ann

Contributors dc:contributor

• Erdman, John W.

• Biology, Molecular
• Health Sciences, Nutrition

Statement dc:rights

• Copyright 1995 Swartz, Deborah Ann

Language dc:language

eng

Identifier

AAI9624505
(UMI)AAI9624505

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/19491