Biochemical and genetic analysis of a fission yeast ribonucleoprotein homologous to signal recognition particle

Protein translocation across the endoplasmic reticulum of higher cells is dependent on a ribonucleoprotein complex known as signal recognition particle (SRP). SRP is composed of six polypeptides and one molecule of 7SL RNA. A homolog of mammalian SRP RNA was found in the fission yeast, Schizosaccharomyces pombe. The gene encoding the S. pombe RNA was cloned by screening a genomic library. Sequence analysis shows that the two RNAs are structurally similar. Disruption of the chromosomal copy of the single copy 7SL gene is lethal to the haploid yeast, demonstrating that the RNA is essential for cell viability. Since secretion is an essential function of the cell, this observation is consistent with its proposed role in this process.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Brennwald, Patrick Joseph

Contributors dc:contributor

• Wise, Jo Ann

• Biology, Molecular
• Biology, Microbiology
• Chemistry, Biochemistry

Statement dc:rights

• Copyright 1990 Brennwald, Patrick Joseph

Language dc:language

eng

Identifier

AAI9026145
(UMI)AAI9026145

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/19215