Two-photon fluctuation correlation spectroscopy: Method and applications to protein aggregation and intracellular diffusion

Fluctuation correlation spectroscopy (FCS) is an experimental technique for the study of hydrodynamics and molecular interactions of microscopic systems. The method monitors the statistical behavior of spontaneous equilibrium fluctuations in number occupation of a fluorescent species in a subvolume of a sample. Microscopic models have been developed which relate observed statistical fluctuations in fluorescence observables to physical quantities of interest. This thesis presents the first application of two-photon molecular excitation to FCS. Two-photon excitation offers several advantages over one-photon excitation for FCS experiments. These advantages are discussed, and model systems are studied to calibrate and characterize the capabilities of the technique. The theoretical framework necessary to recover physical parameters from observed fluctuation spectra is also presented.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Physics

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Berland, Keith Michael

Contributors dc:contributor

• Debrunner, Peter G.

• Physics, Optics
• Biophysics, General

Statement dc:rights

• Copyright 1995 Berland, Keith Michael

Language dc:language

eng

Identifier

AAI9624286
(UMI)AAI9624286

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/19158