{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/19158"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/19158","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Two-photon fluctuation correlation spectroscopy: Method and applications to protein aggregation and intracellular diffusion","abstract":"Fluctuation correlation spectroscopy (FCS) is an experimental technique for the study of hydrodynamics and molecular interactions of microscopic systems. The method monitors the statistical behavior of spontaneous equilibrium fluctuations in number occupation of a fluorescent species in a subvolume of a sample. Microscopic models have been developed which relate observed statistical fluctuations in fluorescence observables to physical quantities of interest. This thesis presents the first application of two-photon molecular excitation to FCS. Two-photon excitation offers several advantages over one-photon excitation for FCS experiments. These advantages are discussed, and model systems are studied to calibrate and characterize the capabilities of the technique. The theoretical framework necessary to recover physical parameters from observed fluctuation spectra is also presented.","abstract_html":"Fluctuation correlation spectroscopy (FCS) is an experimental technique for the study of hydrodynamics and molecular interactions of microscopic systems. The method monitors the statistical behavior of spontaneous equilibrium fluctuations in number occupation of a fluorescent species in a subvolume of a sample. Microscopic models have been developed which relate observed statistical fluctuations in fluorescence observables to physical quantities of interest. This thesis presents the first application of two-photon molecular excitation to FCS. Two-photon excitation offers several advantages over one-photon excitation for FCS experiments. These advantages are discussed, and model systems are studied to calibrate and characterize the capabilities of the technique. The theoretical framework necessary to recover physical parameters from observed fluctuation spectra is also presented.","abstract_has_math":false,"creators":["Berland, Keith Michael"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Physics","degree_department":null,"school":null,"contributors":["Debrunner, Peter G."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T11:58:43Z","date_published":"2011-05-07T11:58:43Z","updated_at":"2026-07-22T22:25:12Z","subjects":["Physics, Optics","Biophysics, General"],"languages":["eng"],"rights":["Copyright 1995 Berland, Keith Michael"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9624286","(UMI)AAI9624286"],"render_values":[{"text":"AAI9624286","href":null,"code":true},{"text":"(UMI)AAI9624286","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/19158","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Debrunner, Peter G."]},{"key":"dc:creator","label":"Author","values":["Berland, Keith Michael"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T11:58:43Z","10000-01-01","1995"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physics"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Physics, Optics","Biophysics, General"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1995 Berland, Keith Michael"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9624286","(UMI)AAI9624286","http://hdl.handle.net/2142/19158"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Fluctuation correlation spectroscopy (FCS) is an experimental technique for the study of hydrodynamics and molecular interactions of microscopic systems. The method monitors the statistical behavior of spontaneous equilibrium fluctuations in number occupation of a fluorescent species in a subvolume of a sample. Microscopic models have been developed which relate observed statistical fluctuations in fluorescence observables to physical quantities of interest. This thesis presents the first application of two-photon molecular excitation to FCS. Two-photon excitation offers several advantages over one-photon excitation for FCS experiments. These advantages are discussed, and model systems are studied to calibrate and characterize the capabilities of the technique. The theoretical framework necessary to recover physical parameters from observed fluctuation spectra is also presented.","There are two biophysical applications proposed and discussed in this thesis. The first is the study of particle mobilities and intracellular diffusion. Measurements of particle diffusion in the cytoplasm of live cells are presented. The second application is the detection of protein aggregation in solution using two-photon scanning FCS. Measurements are shown demonstrating the potential to study aggregation equilibria at low protein concentrations, and the dissociation reactions of several oligomeric proteins are studied. The possibility to measure dissociation kinetics is also demonstrated.","Made available in DSpace on 2011-05-07T11:58:43Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9624286.pdf: 2924273 bytes, checksum: ce4baea791915dbcb9b3a6c13b98d216 (MD5) Previous issue date: 1995","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:35:03Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:13:39-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Two-photon fluctuation correlation spectroscopy: Method and applications to protein aggregation and intracellular diffusion"]}]}],"canonical_facts":{"dc:contributor":["Debrunner, Peter G."],"dc:creator":["Berland, Keith Michael"],"dc:date":["2011-05-07T11:58:43Z","10000-01-01","1995"],"dc:description":["Fluctuation correlation spectroscopy (FCS) is an experimental technique for the study of hydrodynamics and molecular interactions of microscopic systems. The method monitors the statistical behavior of spontaneous equilibrium fluctuations in number occupation of a fluorescent species in a subvolume of a sample. Microscopic models have been developed which relate observed statistical fluctuations in fluorescence observables to physical quantities of interest. This thesis presents the first application of two-photon molecular excitation to FCS. Two-photon excitation offers several advantages over one-photon excitation for FCS experiments. These advantages are discussed, and model systems are studied to calibrate and characterize the capabilities of the technique. The theoretical framework necessary to recover physical parameters from observed fluctuation spectra is also presented.","There are two biophysical applications proposed and discussed in this thesis. The first is the study of particle mobilities and intracellular diffusion. Measurements of particle diffusion in the cytoplasm of live cells are presented. The second application is the detection of protein aggregation in solution using two-photon scanning FCS. Measurements are shown demonstrating the potential to study aggregation equilibria at low protein concentrations, and the dissociation reactions of several oligomeric proteins are studied. The possibility to measure dissociation kinetics is also demonstrated.","Made available in DSpace on 2011-05-07T11:58:43Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9624286.pdf: 2924273 bytes, checksum: ce4baea791915dbcb9b3a6c13b98d216 (MD5) Previous issue date: 1995","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:35:03Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:13:39-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9624286","(UMI)AAI9624286","http://hdl.handle.net/2142/19158"],"dc:language":["eng"],"dc:rights":["Copyright 1995 Berland, Keith Michael"],"dc:subject":["Physics, Optics","Biophysics, General"],"dc:title":["Two-photon fluctuation correlation spectroscopy: Method and applications to protein aggregation and intracellular diffusion"],"dc:type":["text"],"thesis:degree_discipline":["Physics"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:12Z"}