Biochemical characterization of nitrate transport at the plasma membrane of plant cells using isolated vesicles

A modified method for the production and purification of $\sp{36}$ClO$\sb3\sp{-}$ in high yield is described. This procedure, involving the elctrolytic oxidation of $\sp{36}$Cl in a cell with simple electrode design and purification of the electrolysis products ($\sp{36}$Cl, $\sp{36}$ClO$\sb3\sp{-}$ and $\sp{36}$ClO$\sb4\sp{-}$) by aqueous column chromatography in Sephadex G-10, allows for the recovery of about 80% of the initial radioactivity as $\sp{36}$ClO$\sb3\sp{-}$. This radiolabeled anion was used as a tracer for nitrate in membrane transport studies with isolated plasma membrane vesicles.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Crop Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Ruiz-Cristin, Jose-Luis

Contributors dc:contributor

• Briskin, Donald P.

• Agriculture
• Chemistry
• Agronomy
• Biochemistry

Statement dc:rights

• Copyright 1990 Ruiz-Cristin, Jose-Luis

Language dc:language

eng

Identifier

AAI9026310
(UMI)AAI9026310

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/19009