{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/19009"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/19009","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Biochemical characterization of nitrate transport at the plasma membrane of plant cells using isolated vesicles","abstract":"A modified method for the production and purification of $\\sp{36}$ClO$\\sb3\\sp{-}$ in high yield is described. This procedure, involving the elctrolytic oxidation of $\\sp{36}$Cl in a cell with simple electrode design and purification of the electrolysis products ($\\sp{36}$Cl, $\\sp{36}$ClO$\\sb3\\sp{-}$ and $\\sp{36}$ClO$\\sb4\\sp{-}$) by aqueous column chromatography in Sephadex G-10, allows for the recovery of about 80% of the initial radioactivity as $\\sp{36}$ClO$\\sb3\\sp{-}$. This radiolabeled anion was used as a tracer for nitrate in membrane transport studies with isolated plasma membrane vesicles.","abstract_html":"A modified method for the production and purification of $\\sp{36}$ClO$\\sb3\\sp{-}$ in high yield is described. This procedure, involving the elctrolytic oxidation of $\\sp{36}$Cl in a cell with simple electrode design and purification of the electrolysis products ($\\sp{36}$Cl, $\\sp{36}$ClO$\\sb3\\sp{-}$ and $\\sp{36}$ClO$\\sb4\\sp{-}$) by aqueous column chromatography in Sephadex G-10, allows for the recovery of about 80% of the initial radioactivity as $\\sp{36}$ClO$\\sb3\\sp{-}$. This radiolabeled anion was used as a tracer for nitrate in membrane transport studies with isolated plasma membrane vesicles.","abstract_has_math":true,"creators":["Ruiz-Cristin, Jose-Luis"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Crop Sciences","degree_department":null,"school":null,"contributors":["Briskin, Donald P."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T11:54:12Z","date_published":"2011-05-07T11:54:12Z","updated_at":"2026-07-22T22:25:12Z","subjects":["Agriculture","Chemistry","Agronomy","Biochemistry"],"languages":["eng"],"rights":["Copyright 1990 Ruiz-Cristin, Jose-Luis"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9026310","(UMI)AAI9026310"],"render_values":[{"text":"AAI9026310","href":null,"code":true},{"text":"(UMI)AAI9026310","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/19009","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Briskin, Donald P."]},{"key":"dc:creator","label":"Author","values":["Ruiz-Cristin, Jose-Luis"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T11:54:12Z","10000-01-01","1990"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture","Chemistry","Agronomy","Biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1990 Ruiz-Cristin, Jose-Luis"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9026310","(UMI)AAI9026310","http://hdl.handle.net/2142/19009"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["A modified method for the production and purification of $\\sp{36}$ClO$\\sb3\\sp{-}$ in high yield is described. This procedure, involving the elctrolytic oxidation of $\\sp{36}$Cl in a cell with simple electrode design and purification of the electrolysis products ($\\sp{36}$Cl, $\\sp{36}$ClO$\\sb3\\sp{-}$ and $\\sp{36}$ClO$\\sb4\\sp{-}$) by aqueous column chromatography in Sephadex G-10, allows for the recovery of about 80% of the initial radioactivity as $\\sp{36}$ClO$\\sb3\\sp{-}$. This radiolabeled anion was used as a tracer for nitrate in membrane transport studies with isolated plasma membrane vesicles.","A nitrate/chlorate transport activity associated with the plasma membrane vesicles isolated from red beet storage tissue has been identified and characterized. This transport activity is inhibited by the protein modified reagent phenylglyoxal (PGO) and it is mediated by a uniport-type carrier driven by the membrane potential. Given the prevailing conditions of the electrochemical gradient across the membrane of plant cells, this activity seems to correspond with efflux from the intact plant cell. It is proposed that this transport activity is involved in the translocation of nitrate accumulated in the storage tissue to the growing shoot during the second season of this biennial plant.","Nitrate/chlorate transport in maize root plasma membrane vesicles is mediated by a symport-type carrier driven by the proton gradient across the membrane ($\\Delta$pH). This activity seems to correspond to the cell uptake system most likely involved in nitrate acquisition. The protein modifier phenylglyoxal also inhibited this activity. Kinetic studies showed that protons, in addition to acting as the driving force for nitrate/chlorate uptake into the vesicles, changed the affinity of the carrier for the solute (Km) with no effect on Vmax, suggesting an ordered reaction mechanism for the carrier. This mechanism would involve initial H$\\sp{+}$ binding to the carrier followed by the binding of nitrate/chlorate.","Made available in DSpace on 2011-05-07T11:54:12Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9026310.pdf: 5523596 bytes, checksum: f1528c537efcc14feeb0ddc25c61f8ad (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:34:02Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:12:49-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Biochemical characterization of nitrate transport at the plasma membrane of plant cells using isolated vesicles"]}]}],"canonical_facts":{"dc:contributor":["Briskin, Donald P."],"dc:creator":["Ruiz-Cristin, Jose-Luis"],"dc:date":["2011-05-07T11:54:12Z","10000-01-01","1990"],"dc:description":["A modified method for the production and purification of $\\sp{36}$ClO$\\sb3\\sp{-}$ in high yield is described. This procedure, involving the elctrolytic oxidation of $\\sp{36}$Cl in a cell with simple electrode design and purification of the electrolysis products ($\\sp{36}$Cl, $\\sp{36}$ClO$\\sb3\\sp{-}$ and $\\sp{36}$ClO$\\sb4\\sp{-}$) by aqueous column chromatography in Sephadex G-10, allows for the recovery of about 80% of the initial radioactivity as $\\sp{36}$ClO$\\sb3\\sp{-}$. This radiolabeled anion was used as a tracer for nitrate in membrane transport studies with isolated plasma membrane vesicles.","A nitrate/chlorate transport activity associated with the plasma membrane vesicles isolated from red beet storage tissue has been identified and characterized. This transport activity is inhibited by the protein modified reagent phenylglyoxal (PGO) and it is mediated by a uniport-type carrier driven by the membrane potential. Given the prevailing conditions of the electrochemical gradient across the membrane of plant cells, this activity seems to correspond with efflux from the intact plant cell. It is proposed that this transport activity is involved in the translocation of nitrate accumulated in the storage tissue to the growing shoot during the second season of this biennial plant.","Nitrate/chlorate transport in maize root plasma membrane vesicles is mediated by a symport-type carrier driven by the proton gradient across the membrane ($\\Delta$pH). This activity seems to correspond to the cell uptake system most likely involved in nitrate acquisition. The protein modifier phenylglyoxal also inhibited this activity. Kinetic studies showed that protons, in addition to acting as the driving force for nitrate/chlorate uptake into the vesicles, changed the affinity of the carrier for the solute (Km) with no effect on Vmax, suggesting an ordered reaction mechanism for the carrier. This mechanism would involve initial H$\\sp{+}$ binding to the carrier followed by the binding of nitrate/chlorate.","Made available in DSpace on 2011-05-07T11:54:12Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9026310.pdf: 5523596 bytes, checksum: f1528c537efcc14feeb0ddc25c61f8ad (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:34:02Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:12:49-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9026310","(UMI)AAI9026310","http://hdl.handle.net/2142/19009"],"dc:language":["eng"],"dc:rights":["Copyright 1990 Ruiz-Cristin, Jose-Luis"],"dc:subject":["Agriculture","Chemistry","Agronomy","Biochemistry"],"dc:title":["Biochemical characterization of nitrate transport at the plasma membrane of plant cells using isolated vesicles"],"dc:type":["text"],"thesis:degree_discipline":["Crop Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:12Z"}